摘要
续随子(Euphorbia lathyris L.)种子可积累大量油脂且富含油酸,WRINKLED1(WRI1)为AP2/EREBP类转录因子,参与调控脂肪酸的合成及种子发育等生命过程。本研究通过对续随子的全基因组鉴定,获得了11个续随子ElWRI1转录因子基因,命名为ElWRI1-1~ElWRI1-11。结果显示,它们均具有两个AP2结构域;其编码蛋白均属于不稳定亲水蛋白,无跨膜结构;亚细胞定位预测显示所有WRI1蛋白均位于细胞核。表达分析结果表明,ElWRI1-6与ElWRI1-8在种子发育时期的表达量较高,且后者的表达模式与种子油脂积累模式一致。在本氏烟草(Nicotiana benthamiana L.)叶片中异源过表达ElWRI1-8,叶片中总油脂含量增加,且油酸含量显著上升。研究结果表明ElWRI1-8可能是调控续随子种子油脂和油酸生物合成的一个重要转录因子。
Seeds of Euphorbia lathyris L.accumulate a substantial amount of oil enriched with oleic acid.The plant transcription factor WRINKLED1(WRI1)is a member of the AP2/EREBP transcription factor family,which is involved in the regulation of fatty acid synthesis and seed development.Based on the whole genome data of E.lathyris,11 ElWRI1 genes were identified,namely ElWRI1-1–ElWRI1-11,respectively.All identified ElWRI1s contained two AP2 domains.The ElWRI1 proteins were unstable hydrophilic proteins,lacking a transmembrane structure.The 11 ElWRI1 proteins were predicted to localize in the nucleus.Heatmap analysis revealed that ElWRI1-6 and ElWRI1-8 exhibited higher expression levels,with the expression pattern of El-WRI1-8 mirroring that of seed oil accumulation.Heterologous overexpression of ElWRI1-8 in Nicotiana benthamiana L.leaf tissues led to a significant increase in total oil and oleic acid contents in the leaf.This study indicates that ElWRI1-8 is an important transcription factor in regulating biosynthesis of oil and oleic acid in E.lathyris seeds,thus providing a new scientific basis for the high-yield and high-quality breeding of E.lathyris and the development of high-value oil products.
作者
牛听风
葛丽萍
苏云婷
王壮琳
李润植
Niu Ting-Feng;Ge Li-Ping;Su Yun-Ting;Wang Zhuang-Lin;Li Run-Zhi(College of Forestry,Shanxi Agricultural University,Taigu,Shanxi 030801,China;Institute of Molecular Agriculture and Bioenergy,Shanxi Agricultural University,Taigu,Shanxi 030801,China)
出处
《植物科学学报》
CAS
CSCD
北大核心
2023年第4期458-466,共9页
Plant Science Journal
基金
山西省自然科学基金项目(201901D111223)
山西省研究生教育创新项目(J202282045)。
关键词
续随子
转录因子
WRI1基因
油脂合成
叶片瞬时表达
Euphorbia lathyris
Transcription factors
WRI1
Oil synthesis
Leaf transient expression
