摘要
萜类化合物(Terpenoiods)是番茄中一类重要的次生代谢产物,也是受虫害诱导而产生最多的一类挥发物;萜类合成酶基因(terpene synthase,TPS)是萜类生物合成途径中的关键酶。以‘Micro Tom’番茄为材料,利用RT-PCR技术克隆番茄SlTPS7和SlTPS16基因,并对获得的基因序列进行生物信息学分析;利用荧光定量PCR技术检测施氮量为0.38、1.534、7.67、15.34和23.01 mmol/L不同浓度氮素营养液中番茄SlTPS7和SlTPS16基因的表达情况。结果显示,SlTPS7和SlTPS16基因开放阅读框(ORF)分别为1779和1218 bp,编码592和405个氨基酸;均为酸性不稳定亲水蛋白,其结构以α-螺旋(Alpha helix)及无规则卷曲(Random coil)为主,亚细胞预测显示SlTPS7和SlTPS16可能在细胞膜和叶绿体上表达;在不同浓度氮素处理下,SlTPS7和SlTPS16基因表达情况存在显著差异,适量的减氮处理能够促进番茄叶片SlTPS7、SlTPS16的表达,结果可为氮素对番茄挥发性萜类合成酶基因的调控研究提供参考。
Terpenoiods are an important type of secondary metabolites in tomato,and they are also the type of volatiles that are most induced by insect pests;terpene synthase(TPS)is the pathway of terpenoid biosynthesis the key enzyme.Using'Micro Tom'tomato as material,tomato SlTPS7 and SlTPS16 genes were cloned by RT-PCR,and the obtained gene sequences were analyzed by bioinformatics.The expression of SlTPS7 and SlTPS16 genes in tomato nitrogen nutrient solution with different nitrogen concentrations of 0.38,1.534,7.67,15.34 and 23.01 mmol/L was detected by fluorescence quantitative PCR.The results showed that the open reading frame(ORF)of SlTPS7 and SlTPS16 genes were 1779 and 1218 bp,respectively,encoding 592 and 405 amino acids;both were acidic labile hydrophilic proteins,and their structures were alpha helix and random coils,subcellular predictions show that SlTPS7 and SlTPS16 may be expressed on cell membranes and chloroplasts;under different concentrations of nitrogen treatment,the expression of SlTPS7 and SlTPS16 genes are significantly different,and a moderate amount of nitrogen reduction treatment can promote SlTPS7 in tomato leaves.The expression of SlTPS16,the results can provide reference for the regulation of tomato volatile terpenoid synthase genes by nitrogen.
作者
杜志杰
王树彬
何晓丽
杨丹青
赵欢欢
钟媚
钟凤林
Du Zhijie;Wang Shubin;He Xiaoli;Yang Danqing;Zhao Huanhuan;Zhong Mei;Zhong Fenglin(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,350002)
出处
《分子植物育种》
CAS
北大核心
2023年第14期4587-4594,共8页
Molecular Plant Breeding
基金
福建省科技重大专项南方特色设施番茄良种高产技术产业化示范项目(102-K6017201A)
福建省新世纪人才项目(KLa17011A)共同资助。
