摘要
萜类合成酶(terpenoid synthase, TPS)是植物中催化萜类物质合成的关键酶,在植物的生长发育及抗性调节中发挥着重要作用。本研究旨在对番茄萜类合成酶基因家族进行系统的鉴定及分析,为TPS基因的功能研究和利用提供数据参考。基于番茄的全基因组测序结果鉴定TPS基因家族,运用生物信息学方法构建系统发育树,对其基因结构、染色体定位及进化选择压力等进行分析。共鉴定获得31条番茄TPS基因,包括7条未报道基因。系统发育树分析表明其分为5个亚族,且不均匀分布在11条染色体上,呈现串联重复。蛋白理化性质分析表明,番茄TPS基因为酸性的亲水性蛋白。对来源于TPS-a亚家族基因进行Ka/Ks分析,结果表明番茄TPS基因间在进化时主要受纯化选择作用。顺式作用元件分析表明其主要含有光响应,水杨酸响应及防御和应激反应顺式作用元件。通过对基因表达分析发现有18条TPS可表达,其中部分基因在根,茎,叶中均有上调表达。本研究对番茄TPS家族基因进行系统鉴定,预测及进化分析,对萜类合成及调控的研究具有重要的意义。
Terpenoid synthase(TPS) is a key enzyme that catalyzes the synthesis of terpenoids in plants and plays an important role in plant growth and resistance regulation. The aim of this study is to identify and analyze the family of tomato terpenoid synthase genes in a systematic way so as to lay a foundation for the functional research and utilization of TPS genes. The TPS gene family was identified based on the genome-wide sequencing results of tomato, the phylogenetic tree was constructed by bioinformatics method, and its gene structure, chromosome location and evolutionary selection pressure were analyzed. A total of 31 TPS genes were identified, including 7 unreported genes. Phylogenetic tree analysis showed that it was divided into 5 subfamilies and unevenly distributed on 11 chromosomes, showing tandem repeats. The analysis of the physicochemical properties of the protein showed that the TPS gene of tomato was acidic hydrophilic protein. Ka/Ks analysis of gene from TPS-a subfamily showed that TPS gene in tomato were mainly affected by purification and selection during evolution. Analysis of cis-acting elements shows that it mainly contains light response, salicylic acid response, defense and stress response cis-acting elements. Through the analysis of gene expression, 18 TPS genes were found to be expressible, some of which were up-regulated in roots, stems and leaves. In this study, systematic identification, prediction and evolutionary analysis of TPS family genes in tomato are of great significance to the study of terpenoid synthesis and regulation.
作者
杨仕梅
张天缘
丘日光
宋莉
沈奇
Yang Shimei;Zhang Tianyuan;Qiu Riguang;Song Li;Shen Qi(Guizhou Key Lab of Agro-Bioengineering,The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education),Institute of Agro-Bioengineering and College of Life Sciences,Guizhou University,Guiyang,550025;Rapeseed Research In-stitute,Guiyang Academy of Agricultural Sciences,Guiyang,550008)
出处
《分子植物育种》
CAS
CSCD
北大核心
2019年第16期5215-5223,共9页
Molecular Plant Breeding
基金
贵州省科技厅农业攻关项目(黔科合NY字(2016)3052号)
贵州省农业科学院专项资金项目(黔农科院院专项(2016)020号)
贵州省农科院青年基金(黔农科院青年基金(2017)04)共同资助
