摘要
本试验旨在研究饲料中添加黑水虻虫浆(BSFLP)对杂交鳢血清免疫、抗氧化指标和肠道形态、菌群及抗病力的影响。添加0(FM,对照)、2%(BSFLP2)、4%(BSFLP4)和6%(BSFLP6)BSFLP湿物质(分别对应添加0、9、18、27 g/kg BSFLP干物质)配制4种等蛋白质和等脂肪的饲料。选取420尾均质量为(10.84±0.01)g的杂交鳢,随机分成4组,每组3个重复,每个重复35尾,养殖周期为56 d。结果表明:各组间血清丙二醛含量、溶菌酶、过氧化氢酶、碱性磷酸酶、超氧化物歧化酶、谷胱甘肽过氧化物酶活性及总抗氧化能力无显著差异(P>0.05)。与FM组比较,试验组肠绒毛宽度、绒毛高度无显著变化(P>0.05),BSFLP4组肌层厚度、杯状细胞数显著增加(P<0.05)。BSFLP4组肠内容物菌群Shannon指数显著高于FM组(P<0.05),物种多样性增加。BSFLP6组后肠菌群Sobs指数、Chao指数和Ace指数显著高于BSFLP2组(P<0.05)。用舒伯特气单胞菌感染,BSFLP4组死亡率显著低于FM组和BSFLP6组(P<0.05)。综上所述,饲料中添加BSFLP可以改善杂交鳢免疫、抗氧化能力,调节肠道形态和肠道内容物菌群结构、增加菌群多样性进而提高肠道健康,增强抗舒伯特气单胞菌感染的能力。综合来看,杂交鳢饲料中添加18 g/kg BSFLP干物质为宜。
This experiment was to investigate the effects of black soldier fly larvae pulp(BSFLP)on serum immune,and antioxidant indexes,intestinal morphology,intestinal flora,and disease resistance of hybrid snakehead.Four isonitrogen and isolipid diets were prepared by adding 0(FM),2%(BSFLP2),4%(BSFLP4)and 6%(BSFLP6)of BSFLP wet matter(corresponding to the addition of 0,9,18,27 g/kg BSFLP dry matter,respectively).A total of 420 snakeheads with an average body weight of(10.84±0.01)g were randomly divided into 4 groups with 3 replicates per group and 35 hybrid snakehead per replicate.The feeding period was 56 days.The results showed as follows:there were no significant differences in serum ma⁃londialdehyde content,lysozyme,catalase,alkaline phosphatase,superoxide dismutase,glutathione peroxi⁃dase activities and total antioxidant capacity among all groups(P>0.05).Compared with the FM group,the intestinal villus width and villus height in experimental groups had no significant differences(P>0.05),and the muscle layer thickness and goblet cell number in BSFLP4 group were significantly increased(P<0.05).The Shannon index of intestinal contents in BSFLP4 group was significantly higher than that in FM group(P<0.05),and the species diversity was increased.The intestinal flora Sobs index,Chao index and Ace index in BSFLP6 group were significantly higher than those in BSFLP2 group(P<0.05).The mortality in BSFLP4 group was significantly lower than that in FM and BSFLP6 groups after Aeromonas schubertii infection(P<0.05).In conclusion,dietary addition of BSFLP can improve immune antioxidant capacity,regulate intestinal morphology and microbial structure of intestinal contents,increase microbial diversity,improve intestinal func⁃tion,and enhance the ability to resist Aeromonas schubertii infection.It is recommended to add 18 g/kg BSFLP dry matter for the health of hybrid snakehead.
作者
谢雨桐
朱喜锋
李诗洋
黄燕华
刘春
鲁慧杰
王绥涛
王国霞
XIE Yutong;ZHU Xifeng;LI Shiyang;HUANG Yanhua;LIU Chun;LU Huijie;WANG Suitao;WANG Guoxia(Collaborative Innovation Center of Aquatic Sciences,Guangdong Key Laboratory of Animal Breeding and Nutrition,Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China;Guangzhou Fishtech Biotechnology Co.,Ltd.,Guangzhou 510640,China;Innovative Institute of Animal Healthy Breeding,College of Animal Sciences and Technology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2023年第8期5297-5308,共12页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
广东省现代农业产业技术体系创新团队建设项目(2022KJ115,2023KJ115)。
关键词
黑水虻虫浆
杂交鳢
免疫
抗氧化
肠道结构
肠道微生物
舒伯特气单胞菌
larvae pulp
hybrid snakehead
immune
antioxidant
intestinal structure
intestinal microbes
Aeromonas schubertii
