摘要
目的:分离鉴定槲蕨不同部位中功能成分并为其代谢产物生物合成途径提供重要信息。方法:采用非靶向代谢组对槲蕨的根茎、叶柄、叶的代谢组分进行高度富集比较分级。结果:在根茎、叶柄和叶中共鉴定出493个代谢物,通过多元统计分析筛选出98个差异代谢物(DAMs)。其中,四甲氧基黄酮代谢物在槲蕨的叶柄中表达最高,显著高于叶;芹菜素代谢物在槲蕨的叶中表达最高,显著高于根茎和叶柄;其余13个黄酮类代谢物均集中在槲蕨的根茎中,显著高于其他部位。根茎、叶柄和叶的DAMs主要富集在淀粉和蔗糖代谢通路。结论:可为槲蕨不同部位中功能成分的分离鉴定和代谢生物合成途径的解析提供参考。
Objective:To provide important information for separating and identifying functional components in different tissue of Drynaria roosii Nakaike and for elucidating biosynthesis pathways of metabolites of D.roosii.Methods:The non-targeted metabolomics was performed for comparing and grading metabolic components of the rhizome,petiole,and leaf of D.roosii in terms of high enrichment.Results:A total of 493 metabolites were identified in the rhizome,petiole,and leaf,and 98 differential metabolites were selected by the multivariate statistical analysis,Among them,the expression of tetramethoxy flavone in the petiole of D.roosii was the highest,significantly higher than that in the leaf;the expression of apigenin in the leaf of D.roosii was the highest,significantly higher than that in the rhizome and petiole;the other 13 flavonoid metabolites were concentrated in the rhizome of D.roosii,significantly higher than other parts.Differential metabolites of the rhizome,petiole,and leaf were mainly concentrated in the pathway of starch and sucrose metabolism.Conclusion:This study provides a reference for separating and identifying functional components in different tissue of D.roosii and for elucidating biosynthesis pathways of metabolites.
作者
李翠
胡营
杨晓男
雷明
郭晓云
韦筱媚
黄丽容
檀龙颜
缪剑华
LI Cui;HU Ying;YANG Xiao-nan;LEI Ming;GUO Xiao-yun;WEI Xiao-mei;HUANG Li-rong;TAN Long-yan;MIAO Jian-hua(Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement/Guangxi Engineering Research Center of TCM Resource Intelligent Creation,Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China;School of Pharamcy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)
出处
《中国现代中药》
CAS
2023年第7期1564-1574,共11页
Modern Chinese Medicine
基金
国家自然科学基金项目(31860056)
广西创新驱动发展专项(桂科AA18242040)
广西自然科学基金青年科学基金项目(2020GXNSFAA159151)
对发展中国家科技援助项目(KY201904001)
广西科技基地和人才专项(桂科AD22080012)
科技基础资源调查专项(2018FY100700)
广西农业科技创新联盟项目(桂农科盟202211)。
关键词
槲蕨
代谢组
代谢产物
Drynaria roosii Nakaike
metabolomics
metabolite
