摘要
目的探讨兔糖尿病周围神经病变模型的建立方法,并评价超声在兔糖尿病周围神经病变模型中的应用价值。方法将30只雄性新西兰兔随机分为正常对照组(N组,n=6)和造模组(n=24)。造模组按体重65 mg/kg耳缘静脉注射新鲜配制的5%四氧嘧啶,维持其血糖浓度大于16.0 mmol/L 1周以上,随机等分为糖尿病病程1月组(A组)、2月组(B组)和3月组(C组)。N组注射等量生理盐水。分别于1、2、3个月后,利用超声测量A、B、C及N组兔的坐骨神经横截面面积,分析坐骨神经病变前后的超声表现。记录各组兔坐骨神经的神经传导速度,检测后分批取材行病理染色,观察其病理结构的改变。结果造模组24只四氧嘧啶诱导的兔糖尿病模型中2只死亡,1只血糖低于16.0 mmol/L,其余21只成功建立糖尿病模型,成功率为87.5%。超声测得A、B、C组神经横截面面积平均值分别为(2.30±0.13)、(2.90±0.17)、(3.86±0.47)mm^(2),其中B组、C组显著大于N组的(2.03±0.26)mm^(2)(P<0.01)。在四氧嘧啶诱导3个月后,兔坐骨神经传导速度减低,传导速度为(51.14±0.45)m/s,与N组比较差异有统计学意义(P<0.05);A、B与N组比较,动作电位传导速度差异均无统计学意义(P<0.05)。结论低剂量四氧嘧啶诱导兔糖尿病3个月后,坐骨神经出现神经传导速度降低,成功建立糖尿病周围神经病变模型,且造模成功率较高,死亡率低。超声成像可以定量评估兔糖尿病模型坐骨神经的形态变化,可能对糖尿病周围神经病变的诊断具有重要意义。
Objective To investigate the establishment method of a rabbit model for diabetic peripheral neuropathy and evaluate the utility of ultrasound in this model.Methods Thirty male New Zealand rabbits were randomly divided into normal control group(group N,n=6)and model group(n=24).The experimental group was injected with a freshly prepared 5%alloxan solution through the ear vein at a dosage of 65 mg/kg body weight.When the blood glucose concentration of the model group remained above 16.0 mmol/L for more than a week,the participants were randomly assigned to the 1-month group(group A),the 2-month group(group B),and the 3-month group(group C).Group N was injected with the same amount of normal saline.After 1 month,2 months,and 3 months,respectively,the cross-sectional area of the sciatic nerve was measured by ultrasound in groups A,B,C,and N.The ultrasound findings before and after sciatic nerve lesions were then analyzed.The nerve conduction velocity of the sciatic nerve was recorded for each group of rabbits.After the measurements,samples were collected in batches for pathological staining to observe changes in the pathological structure.Results Among the 24 rabbits in the alloxan-induced diabetes model,2 died,1 rabbit had a blood sugar level lower than 16.0 mmol/L,and the remaining 21 rabbits successfully developed diabetes models,resulting in a success rate of 87.5%.The average cross-sectional areas of nerves in group A,group B,and group C,as measured by ultrasound,were(2.30±0.13),(2.90±0.17),and(3.86±0.47)mm^(2),respectively.Among them,group B and group C were significantly larger than group N(2.03±0.26)mm^(2)(P<0.01).After 3 months of alloxan induction,the conduction velocity of the rabbit sciatic nerve decreased to(51.14±0.45)m/s,which was significantly different from that of group N(P<0.05).There was no significant difference in action potential conduction velocity between group A,group B,and group N(P<0.05).Conclusion Three months after inducing diabetes in rabbits using low-dose alloxan,the nerve conduc
作者
黄建波
何英
王红
向茜
杨汐静
张静漪
HUANG Jianbo;HE Ying;WANG Hong;XIANG Xi;YANG Xijing;ZHANG Jingyi(Department of Medical Ultrasound,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,China;Animal Experiment Center,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,China)
出处
《重庆医学》
CAS
2023年第15期2261-2266,共6页
Chongqing medicine
关键词
糖尿病模型
四氧嘧啶
周围神经病变
超声诊断
神经传导速度
diabetes model
alloxan
peripheral neuropathy
ultrasound diagnosis
nerve conduction velocity
