摘要
目的建立实验性1型糖尿病大鼠模型,培养不同病程糖尿病来源的骨髓基质细胞(bone marrow mensenchymal stem cells,BMSCs),观察其体外的生长特性。方法采用空腹腹腔一次性注射65mg/kg链脲佐菌素(streptozocin,STZ)建立1型糖尿病大鼠模型,分别在第2周(A组)、第4周(B组)取其四肢长骨BMSCs,进行体外培养和观察,细胞计数并绘制细胞生长曲线图。对照组按照体重注射柠檬酸缓冲液。结果一次性空腹腹腔注射STZ 1周后,实验组75%的大鼠随机血糖均>16.7mmol/L,出现糖尿病症状,对照组血糖正常,体重无减轻。A组BMSCs与正常组相比,克隆无明显减少,形态类似,各阶段细胞量差别不具有显著性(P>0.05);B组BMSCs与正常组相比,克隆少,胞体略大,生长缓慢,倍增时间延长,抗衰亡能力减弱。与正常组相比,A、B组贴壁功能在24h内差别均不显著(P>0.05)。细胞计数结果显示,第2d至第8d,B组BMSCs比A组和正常组BMSCs均明显减少(P<0.01)。结论 STZ能够成功复制出1型糖尿病大鼠模型。建模4周后糖尿病大鼠BMSCs的增殖能力已受影响,抗衰亡能力减弱。
Objective To establish the type 1 diabetic rat model,to cultivate bone marrow mesenchymal stem cells of different diabetic courses in vitro and to observe their growth characteristic. Methods Streptozocin was injected intraperitoneally into rats to establish type 1 diabetic rat model. BMSCs were harvested from diabetic rats and healthy rats two and four weeks after the establishment of the model respectively and cultured in vitro. Their growth characteristic was observed by light microscope. After purified, BMSCs of P3 were counted from the onset to the 8th day and cell growth curve was drawn. Results One week after injection of streptozocin, 75% of the rats were presented with hyperglycemia and diabetic symptoms. The two week group had similar clone and morphology with the control （ P 〉 0. 05 ）. The four week group had less clone and cells grew slower. The attachment ability of the groups was similar within 24 h （P 〉 0. 05 ）. The cell number of four week group was decreased compared with control and two week group （ P 〈 0. 01 ）. Conclusion BMSCs from the streptozocin -induced type 1 diabetic rats model was established. Four weeks after the establishment of the model, the proliferative and antiapoptosis abilities of diabetic BMSCs were significantly impaired.
出处
《北京口腔医学》
CAS
2013年第6期314-317,共4页
Beijing Journal of Stomatology
基金
北京市自然科学基金(7112060)
北京市卫生系统高层次技术人才培养项目(2011-3-075)
关键词
糖尿病动物
骨髓基质细胞
链脲佐菌素
生长曲线
Diabetic animals
Bone marrow mesenchymal stromal cell
Streptozocin
Growth curve
