摘要
目的探究吡非尼酮对于肝星状细胞活性、自噬以及凋亡的影响。方法使用不同浓度吡非尼酮与自噬激动剂雷帕霉素联合用药处理肝纤维化HSCT6细胞,CCK-8和克隆形成实验检测细胞增殖、划痕实验检测细胞迁移、流式细胞术检测细胞凋亡,蛋白免疫印迹法检测纤维化、自噬及凋亡相关蛋白表达。结果CCK-8和克隆提示随吡非尼酮浓度增加细胞增殖受限,划痕提示吡非尼酮浓度增加细胞迁移受限,蛋白免疫印迹法和流式细胞术检测提示吡非尼酮处理细胞后细胞自噬受限、凋亡增加。联合处理结果提示,自噬激动剂可部分逆转吡非尼酮对HSCT6细胞活性抑制。结论吡非尼酮可抑制肝星状细胞增殖、促进凋亡,可能与其抑制肝星状细胞自噬有关。
Objective To investigate the effect of pirfenidone on the activity,autophagy and apoptosis of hepatic stellate cells(HSC).Methods Hepatic fibrosis HSC T6 cells were treated with combination medication of different concentrations of pirfenidone and rapamycin(an autophagic agonist).CCK8 assay and clone formation assay were used to check the cell proliferation,scratch testing was used to detect the cell migration,flow cytometry was used to determine the cell apoptosis,and Western blot was adopted to test the fibrosis,autophagy and apoptosis-related protein expressions.Results CCK8 assay and clone formation assay tests suggested that cell proliferation was restricted with the increasing concentration of pirfenidone.Scratch testing indicated that cell migration was restricted with the increasing concentration of pirfenidone.Western blot and flow cytometry tests showed that after the treatment with pirfenidone,the autophagy of HSC T6 cells was restricted and the apoptosis of HSC T6 cells was increased.The results of the treatment with combination use of pirfenidone and rapamycin revealed that autophagic agonist(rapamycin)could partially reverse the inhibition effect of pirfenidone on the activity of HSC T6 cells.Conclusion Pirfenidone can inhibit the proliferation and promote apoptosis of HSC T6 cells,which may be related to its inhibition effect on the autophagy of HSC T6 cells.(J Intervent Radiol,2023,32:651-659)
作者
李苒
程芝梅
何慧洲
张帅
王黎洲
周石
LI Ran;CHENG Zhimei;HE Huizhou;ZHANG Shuai;WANG Lizhou;ZHOU Shi(College of Medical Imaging,Guizhou Medical University,Department of Intervention,Affiliated Hospital of Guizhou Medical University,Guiyang,Guizhou Province 550004,China)
出处
《介入放射学杂志》
CSCD
北大核心
2023年第7期651-659,共9页
Journal of Interventional Radiology
基金
国家自然科学基金(81760325)。
关键词
肝纤维化
肝星状细胞
自噬
增殖
凋亡
hepatic fibrosis
hepatic stellate cell
autophagy
proliferation
apoptosis
