摘要
目的研究姜黄素及其类似物抑制CCl4诱导大鼠肝纤维化的作用及机制。方法SD大鼠雄性40只,按体重随机分成模型组,对照组,姜黄素组,H8低、高剂量组,每组8只。模型组,姜黄素组和H8组腹腔注射40%CCl4橄榄油复制肝纤维化模型,8周后姜黄素组,H8低、高剂量组给予相应药物灌胃,同时每周腹腔注射40%CCl4橄榄油一次,共4周。处死大鼠后,检测血液生化指标,肝组织HE和Masson染色观察病理形态学变化,QPCR测Col1、纤连蛋白(FN)、转化生长因子-β(TGF-β)、α-平滑肌肌动蛋白(α-SMA)mRNA。结果模型组谷丙转氨酶(ALT)、谷草转氨酶(AST)、血清谷氨酰转肽酶(GGT)、肝脏系数、Col1、FN、TGF-β、α-SMA mRNA显著高于对照组,差异有高度统计学意义(P<0.01)。姜黄素组,H8低、高剂量组血清ALT、AST、GGT、肝脏系数、Col1、FN、TGF-β、α-SMA mRNA显著低于模型组,差异有统计学意义(P<0.05)。结论姜黄素及其类似物H8对CCl4诱导的肝纤维化大鼠有保护作用,且H8作用优于姜黄素。
Objective To study the effect and mechanism of curcumin and its analogues on CC14 induced hepatic fibrosis in rats.Methods Forty male Wistar rats were randomly divided into model,normal curcumin,H8 low and high dose groups,with 8 rats in each group according to body weight.The model group,curcumin group and H8 group were intraperitoneally injected with 40% CC14 olive oil to replicate the liver fibrosis model.After 8 weeks,the curcumin group,H8 low and high dose groups were given the corresponding drugs by gavage,and intraperitoneal injection of 40% CCl4 olive oil once for 4 weeks.After sacrificed the rats,blood biochemical indicators were measured,pathological morphological changes were observed by HE and Masson staining of liver tissue,while Coll,fibronectin(FN),transforming growth factor-β(TGF-β),and α-smooth muscle actin(A-SMA) mRNA were measured by QPCR.Results The alanine aminotransferase(ALT),aspartate aminotransferase(AST),serum glutamyl transpeptidase(GGT),liver coefficient,Coll,FN,TGF-β,α-SMA mRNA in the model group were significantly higher than those in the control group,and the differences were highly statistically significant(P <0.01).In the curcumin group,the serum ALT,AST,GGT,liver coefficient,Coll,FN,TGF-β,and α-SMA mRNA in the H8 low and high-dose groups were significantly lower than those in the model group,and the differences were statistically significant(P <0.05).Conclusion Curcumin and its analogue H8 have therapeutic and protective effects on CC14 induced liver fibrosis in rats,and H8 has better effects than curcumin.
作者
王子恺
毛泳彬
孔力
钟星
孙晓雯
吴丹
袁晓环
WANG Zikai;MAO Yongbin;KONG Li;ZHONG Xing;SUN Xiaowen;WU Dan;YUAN Xiaohuan(Mudanjiang Medical University,Heilongjiang Province,Mudanjiang 157011,China)
出处
《中国医药导报》
CAS
2020年第8期13-16,共4页
China Medical Herald
基金
国家级大学生创新创业计划项目(201810229018)。
关键词
姜黄素及其类似物
四氯化碳
肝纤维化
肝脏系数
病理形态学
Curcumin and its analogues
Carbon tetrachloride
Liver fibrosis
Liver coefficient
Pathomorphology
