摘要
目的:研究肿瘤坏死因子α(TNF-α)和叉头框蛋白A2(FOXA2)蛋白在结直肠癌组织中的表达情况,探讨其与结直肠癌患者临床病理指标的关系。方法:收集结直肠癌及配对癌旁正常组织39例,选取同期经结肠镜切除并经病理证实的结直肠腺瘤组织42例。采用免疫组织化学技术检测结直肠组织中TNF-α和FOXA2蛋白的表达情况,Spearman法分析二者表达的相关性,卡方检验分析TNF-α和FOXA2蛋白表达与结直肠癌患者临床病理指标的关系。结果:TNF-α蛋白在正常结直肠黏膜、结直肠腺瘤及结直肠癌组织中的阳性表达率分别为23.08%、50%和76.92%,组间比较的差异有统计学意义(P<0.05);FOXA2蛋白在正常结直肠黏膜、结直肠腺瘤及结直肠癌组织中的阳性表达率分别为23.08%、52.38%和79.49%,组间比较的差异有统计学意义(P<0.05)。TNF-α蛋白表达在不同直径、组织学类型腺瘤组织间的差异有统计学意义(P<0.05),FOXA2蛋白的表达在不同直径、形状类型腺瘤组织间的差异有统计学意义(P<0.05)。在结直肠癌组织中,TNF-α蛋白表达在不同分化程度、浸润深度、淋巴结转移及TNM分期组间比较的差异均有统计学意义(P<0.05),FOXA2蛋白表达在不同分化程度、淋巴结转移、TNM分期组间比较的差异均有统计学意义(P<0.05)。结直肠癌组织中TNF-α和FOXA2表达存在正相关,相关系数r_(s)=0.475(P<0.05)。结论:TNF-α和FOXA2蛋白在正常肠黏膜、结直肠腺瘤及结直肠癌组织中的阳性表达率呈逐渐上升趋势,提示TNF-α、FOXA2蛋白与结直肠癌的发生、发展密切相关。肿瘤分化程度越差,浸润深度越深,伴淋巴结转移率越高及TNM分期越晚,TNF-α蛋白阳性表达率越高,提示TNF-α蛋白可能在结直肠癌的发生发展过程中起促进作用。肿瘤分化程度越差,淋巴结转移率越高及TNM分期越晚,FOXA2蛋白阳性表达率越高,提示FOXA2蛋白可能在结直肠癌的发生发展过程中�
OBJECTIVE:To investigate relationships between expression of TNF-αand FOXA2 proteins in colorectal cancer tissues,and clinicopathologic indexes in the patients.METHODS:Thirty-nine cases of colorectal cancer and paired paracancer normal tissues(more than 5 cm from the tumor margin)were collected,and 42 cases of colorectal adenoma tissues which were confirmed by pathology and colonoscopy were selected.Immunohistochemistry was used to determine expression of TNF-αand FOXA2 proteins in colorectal tissue,using the Spearman method for evaluation.The Chi-square test was used to analyze relationships between the expression of TNF-αand FOXA2 protein,and clinicopathologic indexes in the colorectal cancer patients.RESULTS:The positive expression rates for the TNF-αprotein in normal colorectal mucosa,colorectal adenoma and colorectal cancer tissues were 23.08%,50%and 76.92%,respectively,and the differences among the groups were statistically significant.The positive expression rates for the FOXA2 protein in the three tissues were 23.08%,52.38%and 79.49%,respectively,and the differences were statistically significant.There were statistically significant differences in expressions of the TNF-αand of the FOXA2 proteins among different diameter and histological types of adenomas(P<0.05).In colorectal cancer tissues,there were statistically significant differences in TNF-αprotein expression among different degrees of differentiation,depth of invasion,lymph node metastasis and TNM staging,while there were statistically significant differences in FOXA2 protein expression among different degrees of differentiation,lymph node metastasis and TNM staging(P<0.05).The expressions of TNF-αand FOXA2 were positively correlated with r_(s)=0.475(P<0.05).CONCLUSION:The positive expression rates of TNF-αand FOXA2 proteins were different in normal intestinal mucosa,colorectal adenoma and colorectal cancer tissues,with gradually increasing trend,suggesting that TNF-αand FOXA2 proteins were closely related to the occurrence and developmen
作者
黄梦盼
王学红
芦永福
HUANG Mengpan;WANG Xuehong;LU Yongfu(Graduate School of Qinghai University,Xining 810016;Department of Gastroenterology,Affiliated Hospital of Qinghai University,Xining 810012,Qinghai,China)
出处
《癌变．畸变．突变》
CAS
2023年第4期296-301,324,共7页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
青海大学附属医院重点专科项目(青大附医政字[2019]195号)。
