摘要
目的:观察宫颈鳞状细胞癌(SCC)及鳞状上皮内病变组织中β连环蛋白(β-catenin)、淋巴增强子结合蛋白1(LEF1)的表达并探讨其临床意义。方法:选取宫颈SCC组织55例、低级别鳞状上皮内病变(low grade squamous intraepithelial lesion,LSIL)组织42例、高级别鳞状上皮内病变(high grade squamous intraepithelial lesion,HSIL)组织34例、正常宫颈组织21例,对上述组织进行β-catenin、LEF1免疫组化检测,分析两者在宫颈鳞状上皮病变中的表达、二者与病变的相关性及其与SCC临床病理参数的关系。结果:SCC、HSIL、LSIL、正常宫颈组织中β-catenin细胞质表达率分别为32.7%、29.4%、23.8%、4.8%,差异有统计学意义(P=0.003);在以上病变中,β-catenin细胞核表达率分别是41.8%、32.4%、28.6%、9.5%,差异有统计学意义(P=0.002);LEF1阳性率分别为69.0%、50.0%、26.2%、9.5%,差异有统计学意义(P<0.001)。β-catenin的异常表达与SCC分化程度(P=0.036)、淋巴结转移(P=0.033)相关,LEF1的表达与FIGO分期(P=0.020)及淋巴结转移(P=0.024)相关。β-catenin(r s=0.375,P<0.001)及LEF1(r s=0.443,P<0.001)的表达情况与宫颈病变发展呈正相关,β-catenin与LEF1的表达在各组病变中呈正相关(r s=0.403,P<0.001)。结论:宫颈鳞状细胞癌及其癌前病变中β-catenin和LEF1蛋白异常表达,并与宫颈鳞状细胞癌的分化、淋巴结转移及FIGO分期相关。
Objective:To investigate the expression and clinical significance ofβ-catenin and LEF1 in cervical squamous cell carcinoma(SCC)and squamous intraepithelial lesions.Methods:55 cases of cervical SCC tissues,42 cases of LSIL tissues,34 cases of HISL tissues and 21 cases of normal cervical tissues were enrolled.Immunohistochemical analysis ofβ-catenin and LEF1 was performed.The expression ofβ-catenin and LEF1 in cervical lesions,the correlation with cervical lesions and the relationship with clinicopathological parameters were analyzed.Results:The expression rates ofβ-catenin cell cytoplasm in SCC,HSIL,LSIL and normal cervical tissues were 32.7%,29.4%,23.8%and 4.8%,respectively,and the difference was statistically significant(P=0.003).The nuclear expression rates ofβ-catenin were 41.8%,32.4%,28.6%and 9.5%,respectively,with statistical significance(P=0.002).The positive rate of LEF1 was 69.0%,50.0%,26.2%and 9.5%,respectively,and the difference was statistically significant(P<0.001).The abnormal expression ofβ-catenin was correlated with the degree of SCC differentiation(P=0.036)and lymph node metastasis(P=0.033).The expression of LEF-1 was correlated with the FIGO staging(P=0.020)and lymph node metastasis(P=0.024).β-catenin(r s=0.375,P<0.001)and LEF1(r s=0.443,P<0.001)was positively correlated with the development of cervical lesions.LEF1 andβ-catenin expression were positively correlated in each group(r s=0.403,P<0.001).Conclusion:The expression ofβ-catenin and LEF1 is increased in cervical squamous cell carcinoma and cervical precancerous lesions,and is associated with the differentiation,lymph node metastasis and FIGO staging of cervical squamous cell carcinoma.
作者
汪钊
王艳峰
关兵兵
WANG Zhao;WANG Yanfeng;GUAN Bingbing(Department of Pathology,Beidahuang Industry Group General Hospital,Heilongjiang Harbin 150088,China)
出处
《现代肿瘤医学》
CAS
北大核心
2023年第16期3061-3065,共5页
Journal of Modern Oncology
