摘要
目的:探讨白细胞介素6(IL-6)和β连环蛋白(β-catenin)在口腔鳞癌(OSCC)中的表达及临床病理学意义。方法:应用化学发光分析法检测110例OSCC患者和109例健康对照者的血清IL-6浓度;应用免疫组织化学检测68例接受手术治疗的OSCC患者肿瘤标本及6例正常黏膜组织中IL-6和β-catenin的表达水平,分析其与临床病理参数之间的相关性及预后价值。采用SPSS 22.0软件包对数据进行统计学分析。结果:化学发光分析显示OSCC患者血清IL-6含量显著增高(P<0.001)。免疫组织化学染色显示,IL-6在OSCC组织中高表达,与颈淋巴结转移(P=0.017)、病理分化程度(P=0.014)、复发和远处转移(P=0.048)显著相关。Kaplan-Meier生存分析表明,IL-6高表达的OSCC患者预后不良。多因素Cox回归分析表明,IL-6高表达是OSCC患者的独立预后危险因素(P<0.05)。β-catenin高表达与病理分化有关(P=0.006),与OSCC患者的预后较差有关。Spearman相关性分析显示,IL-6与β-catenin在OSCC中的表达呈显著正相关(P<0.001)。结论:血清IL-6可作为诊断OSCC的生物标志物,组织中IL-6和β-catenin的表达可以作为评估OSCC患者预后不良的标志物。
PURPOSE:To investigate the expression of interleukin-6(IL-6)andβ-catenin in oral squamous cell carcinoma(OSCC)and its clinicopathological significance.METHODS:The serum IL-6 concentration in 110 OSCC patients and 109 healthy controls were determined by chemiluminescence analysis.IL-6 andβ-catenin expression levels in 68 tumor specimens of OSCC patients undergoing surgical treatment and 6 normal mucosal tissues were determined by immunohistochemistry method.The correlation between IL-6 andβ-catenin and clinicopathological parameters and their prognostic value were analyzed.SPSS 22.0 software package was used for data analysis.RESULTS:Chemiluminescence method showed that the serum IL-6 content of OSCC patients was significantly increased(P<0.001).Immunohistochemical results demonstrated that high expression of IL-6 in OSCC tissues was remarkably associated with cervical lymph node metastasis(P=0.017),pathological differentiation(P=0.014),recurrence and distant metastasis(P=0.048).OSCC patients with high IL-6 expression showed a poor prognosis by Kaplan-Meier survival analysis.Multivariate Cox regression analysis showed that high expression of IL-6 was an independent risk factor affecting the prognosis of patients with OSCC(P<0.05).β-catenin hyperexpression was associated with pathological differentiation(P=0.006)and overall poor survival in OSCC patients.Spearman correlation analysis showed a positive correlation between IL-6 andβ-catenin expression in OSCC(P<0.001).CONCLUSIONS:Serum IL-6 is expected to be a biomarker for detection of OSCC,and IL-6 andβ-catenin expression in tumour tissues can be used as markers to evaluate the poor prognosis of OSCC.
作者
董淋升
唐进
程维
冉婧璇
刘兆博
李雅冬
DONG Lin-sheng;TANG Jin;CHENG Wei;RAN Jing-xuan;LIU Zhao-bo;LI Ya-dong(Department of Oral and Maxillofacial Surgery,The First Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China)
出处
《上海口腔医学》
CAS
北大核心
2023年第3期280-286,共7页
Shanghai Journal of Stomatology
基金
重庆市教育委员会资助项目(15SKG032)
重庆市科学技术委员会自然科学基金(cstc2018jcyjAX07-63)
重庆市卫生与计划生育委员会医学高端后备人才培养项目(2017-HBRC004)。
