摘要
[目的]探讨宫颈癌患者血清miR-153及miR-142-3p的表达及其在宫颈癌诊断和预后评估中的价值。[方法]选取2014年1月至2017年3月收治的宫颈癌患者142例,采用实时荧光定量聚合酶链反应(RT-PCR)检测宫颈癌患者血清miR-153及miR-142-3p表达水平。绘制ROC曲线分析miR-153及miR-142-3p对宫颈癌诊断的截断值,并以此为标准将宫颈癌患者分为高表达和低表达,对两组临床病理特征进行比较。采用多因素Cox回归模型分析影响宫颈癌患者术后预后不良的危险因素。[结果]宫颈癌组血清miR-153 (0.76±0.38 vs1.92±0.95)及miR-142-3p(2.14±1.06 vs 8.15±3.20)表达水平明显低于对照组(P<0.01)。血清miR-153及miR-142-3p表达水平诊断宫颈癌的最佳截断值分别为1.31和4.93,AUC分别为0.817(95%CI:0.755~0.878)和0.850(95%CI:0.791~0.912)。血清miR-153及miR-142-3p低表达与宫颈癌患者的临床分期、分化程度、浸润深度、淋巴结转移及脉管浸润有关(P<0.05)。miR-153及miR-142-3p低表达与宫颈癌患者生存期短有关(P<0.01)。多因素Cox回归模型分析显示,临床分期、浸润深度、淋巴结转移、miR-153<1.32及miR-142-3p<4.93是影响宫颈癌患者预后不良的独立危险因素。[结论]血清miR-153及miR-142-3p表达水平在宫颈癌患者中明显下调,miR-153及miR-142-3p有望作为宫颈癌诊断及预后评估的生物学标志物。
[Objective] To investigate the expression of serum miR-153 and miR-142-3p in patients with cervical cancer and their diagnostic and prognostic value. [Methods] A total of 142 patients with cervical cancer admitted to our hospital from January 2014 to March 2017 were selected,and the expression levels of serum miR-153 and miR-142-3p in patients with cervical cancer were detected by real-time fluorescence quantitative PCR. ROC curve was drawn to analyze the truncation value of miR-153 and miR-142-3p in the diagnosis of cervical cancer. Based on this criterion,patients with cervical cancer were divided into high miR-153 and miR-142-3p groups and low miR-153 and miR-142-3p groups. The clinicopathological characteristics of the two groups were compared. Multivariate Cox regression models were used to analyze the risk factors affecting poor prognosis of patients with cervical cancer. [Results] The serum levels of miR-153(0.76±0.38 vs 1.92±0.95) and miR-142-3p(2.14±1.06 vs 8.15±3.20) in cervical cancer group were significantly lower than that in control group(P<0.01). The optimal cut-off values of serum miR-153 and miR-142-3p in the diagnosis of cervical cancer were 1.31 and 4.93,and the AUC were 0.817(95%CI:0.755~0.878) and 0.850(95%CI:0.791~0.912),respectively. The low expression of serum miR-153 and miR-142-3p were associated with clinical stage,differentiation degree,depth of invasion,lymph node metastasis and vascular invasion of cervical cancer(P<0.05).The low expression of miR-153 and miR-142-3p were related to the short survival of patients with cervical cancer(P<0.01). Multivariate Cox regression analysis showed that clinical stage,depth of invasion,lymph node metastasis,miR-153<1.32 and miR-142-3p<4.93 were independent risk factors for poor prognosis of cervical cancer patients. [Conclusion] The expression levels of serum miR-153 and miR-142-3p are significantly down-regulated in patients with cervical cancer,and miR-153 and miR-142-3p are expected to be biomarkers for the diagnosis and prognosis of cervic
作者
苏红娥
钟庆吉
符春丽
薛万兴
林元
邓森灵
SU Hong-e;ZHONG Qing-ji;FU Chun-Li;XUE Wan-xing;LIN Yuan;DENG Sen-ling(Central Hospital of Western Hainan,Nada 571799,China)
出处
《肿瘤学杂志》
CAS
2020年第11期972-976,共5页
Journal of Chinese Oncology
