摘要
目的:探讨醒脑开窍针刺法治疗结合康复训练改善缺血性卒中急性期大鼠神经损伤的效应及与Toll样受体(TLRs)通路相关的机制。方法:将大鼠随机分为假手术组和大脑中动脉阻塞(MCAO)组,术后根据神经功能评分将符合条件的MCAO大鼠再分为模型组和治疗组。治疗组于造模后24 h给予针刺治疗及康复训练,连续14 d。采用Zausinger评分评价动物的神经功能,行为学测试评价动物的运动功能,氯化三苯基四氮唑(TTC)染色法检测脑梗死体积,反转录聚合酶链式反应(RT-PCR)、蛋白质免疫印迹法(Western Blot)检测梗死边缘区内TLR4/胞内髓样分化蛋白88(MyD88)/核因子κB(NF-κB)通路mRNA和蛋白表达,酶联免疫吸附法(ELISA)检测同区域内白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)水平。结果:治疗组24 h的神经功能评分最低,其后逐渐升高,组内比较差异有统计学意义(P<0.01);从第3天开始,治疗组神经功能评分与同期模型组相比差异有统计学意义(P<0.05)。行为学测试结果表明,造模后24 h,治疗组转棒成绩和模型组无明显差异,而与假手术组比较差异有统计学意义(P<0.05);其后各时间点,治疗组成绩逐渐提高,从造模后3 d开始,与同期模型组相比差异有统计学意义(P<0.05)。模型组脑梗死体积大于假手术组(P<0.01);治疗组脑梗死体积低于模型组(P<0.01)。模型组TLR4、MyD88、NF-κB mRNA和蛋白表达水平以及IL-1β、TNFα水平较假手术组升高(P<0.05);治疗组mRNA、蛋白表达及炎性因子水平降低,与模型组比较差异有统计学意义(P<0.05)。结论:针刺治疗结合康复训练能够能下调TLR4/MyD88/NF-κB通路活性及相关炎性因子水平,进而改善神经元存活微环境,减轻神经缺血损伤。
Objective:To explore the effect of Xingnao Kaiqiao acupuncture combined with rehabilitation training on improving nerve injury in rats with acute ischemic stroke and the mechanism related to Toll like receptor(TLR4)pathway.Methods::The rats were randomly divided into sham operation group and middle cerebral artery occlusion(MCAO)group.After operation,the eligible MCAO rats were divided into model group and treatment group according to neurological function scores.The rats in treatment group were given acupuncture treatment and rehabilitation training 24 hours after modeling for 14 consecutive days.The Zausinger score was used to evaluate neurological function.The behavioral test was used to evaluate the motor function.The triphenyltetrazolium chloride(TTC)staining was used to detect the cerebral infarct volume.Reverse transcription-polymerase chain reaction(RT-PCR)and Western Blot methods were used to detect TLR4/myeloid differentiation protein 88(MyD88)/nuclear factor kappa-B(NF-κB)pathway mRNA and protein expression in the marginal area of infarction.Enzyme linked immunosorbent assay(ELISA)method was used to detecte interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)levels in the same area.Results:The neurological function score of the treatment group was the lowest at 24 hours,and then gradually increased.The difference within the group was significant(P<0.01);from day 3,the neurological function score of the treatment group was significantly different the model group in the same period(P<0.05).The behavioral test results showed that 24 hours after modeling,the performance of the treatment group was not significantly different compared with that of the model group,but was significantly different from the sham operation group;thereafter,at each time point,the performance of the treatment group gradually improved,from 3 days after modeling,compared with the model group during the same period,the difference was significant(P<0.05).The volume of cerebral infarction in the model group was significantly lar
作者
全宏娟
何旭丽
夏明万
何勇
QUAN Hongjuan;HE Xuli;XIA Mingwan;HE Yong(First People′s Hospital of Chenzhou,Chenzhou 423000,Hunan,China)
出处
《中西医结合心脑血管病杂志》
2023年第14期2607-2612,共6页
Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
基金
湘南学院自然科学项目(No.2019XJ65)。
关键词
缺血性卒中
醒脑开窍针刺法
康复训练
炎症
TOLL样受体
核因子-ΚB
ischemic stroke
Xingnao Kaiqiao acupuncture method
rehabilitation training
inflammation
Toll like receptor
nuclear factor kappa-B
