摘要
目的观察康脑液2号对大鼠局灶性脑缺血再灌注损伤后血清及脑组织中NO、NOS含量的影响,并探讨其可能的作用机制。方法将大鼠随机分为假手术组,模型组,康脑液2号组,依达拉奉组。采用线栓法制作大鼠脑缺血再灌注模型。脑缺血再灌注24h后测各组大鼠血清及脑组织NO含量和NOS活性,光镜下观察脑组织病理变化。结果与假手术组相比,大鼠脑缺血2h再灌注24h血清及脑组织中NO含量及NOS活性显著升高。与模型组相比,康脑液2组大鼠血清及脑组织中NO含量和NOS活性显著降低。结论康脑液2号能够减轻大鼠脑缺血再灌注损伤,作用机制可能与抑制NO、NOS表达有关。
Objective：To observe the effect of Kangnaoye-2 on the contents of NO and NOS in serum and brain tissue of rats with cerebral ischemia-reperfusion injury and to investigate the mechanism of Kangnaoye-2.Methods：Rats were randomly divided into the sham operation group,the model group,the Kangnaoye-2 group and the edaravone group.After being established by the MCAO method,the NO and the NOS of rats were detected after the ischemia-reperfusion for 24h.The brain tissue pathology change was observed by the light microscope.Results：Compared with the sham operation group,the NO content and the NOS activity were higher significantly than those with ischemia for 2h and reperfusion for 24h.Meanwhile,the NO level and the NOS activity were decreased obviously in the Kangnaoye-2 group when they comparing with the model group.Conclusion：Kangnaoye-2 shows protective effects on cerebral ischemia-reperfusion injury.The mechanism may be ralated to refrain the expression of NO and NOS.
出处
《中国中医急症》
2011年第8期1256-1258,共3页
Journal of Emergency in Traditional Chinese Medicine
基金
河北省卫生厅科研课题(20090591)
