摘要
目的 探讨乳腺癌组织中缺氧诱导因子-1α(HIF-1α)和上皮间质转化(EMT)相关蛋白的表达及其与肿瘤血管生成的关系。方法 免疫组化S-P法检测90例乳腺癌和40例乳腺纤维腺瘤组织中HIF-1α、上皮性钙黏蛋白(E-cadherin)、波形蛋白(Vimentin)、血管内皮生长因子-C(VEGF-C)、微血管密度(MVD)的表达,并分析其表达与乳腺癌临床病理特征的关系。结果 HIF-1α在乳腺癌组织中阳性率为68.9%(62/90),高于乳腺纤维腺瘤的7.5%(3/40)(χ^(2)=41.742,P<0.001)。E-cadherin在乳腺癌组织中的阳性率为70.0%(63/90),低于乳腺纤维腺瘤的95.0%(38/40)(χ^(2)=9.998,P<0.001)。Vimentin在乳腺癌组织中的阳性率为34.4%(31/90),高于乳腺纤维腺瘤的0.0%(0/40)(χ^(2)=18.091,P<0.001)。VEGF-C在乳腺癌组织中的阳性率为46.7%(42/90),高于乳腺纤维腺瘤的0.0%(0/40)(χ^(2)=27.582,P<0.001)。MVD在乳腺癌组织中的阳性率为96.7%(87/90),高于乳腺纤维腺瘤的0.0%(0/40)(χ^(2)=116.902,P<0.001)。HIF-1α表达与乳腺癌组织学分级、TNM分期有关(χ^(2)=8.901,P=0.012;χ^(2)=8.711,P=0.013)。E-cadherin表达与乳腺癌肿块大小、组织学分级有关(χ^(2)=5.981,P=0.050;χ^(2)=6.051,P=0.048)。Vimentin表达与乳腺癌肿块大小、淋巴结转移、TNM分期有关(χ^(2)=6.047,P=0.049;χ^(2)=26.683,P<0.001;χ^(2)=28.651,P<0.001)。VEGF-C与乳腺癌淋巴结转移、TNM分期和分子分型有关(χ^(2)=12.562,P=0.000;χ^(2)=17.803,P<0.001;χ^(2)=13.485,P=0.004)。MVD与乳腺癌肿块大小有关(χ^(2)=8.595,P=0.014)。HIF-1α与E-cadherin表达呈负相关(r=-0.513,P<0.001),与Vimentin、VEGF-C、MVD表达呈正相关(r=0.392,P=0.002;r=0.694,P<0.001;r=0.745,P<0.001)。结论 HIF-1α和EMT相关蛋白可能与乳腺癌的肿瘤血管生成有关,HIF-1α可能通过调控EMT在乳腺癌的侵袭、转移过程中发挥作用。
Objective To investigate the expression of hypoxia-inducible factors-1α(HIF-1α)and epithelial-mesenchymal transition(EMT)related proteins in the breast cancer and their relationship with tumor angiogenesis.Methods The expression of HIF-1α,E-cadherin,Vimentin,vascular endothelial growth factor-C(VEGF-C)and microvessel density(MVD)in 90 cases of breast cancer and 40 cases of breast fibroadenoma were detected by immunohistochemical S-P method,the relationship between their expression and the clinicopathological characteristics of breast cancer was analyzed.Results The positive rate of HIF-1αin the breast cancer was 68.9%(62/90),which was higher than that in the breast fibroadenoma[7.5%(3/40);χ^(2)=41.742,P<0.001].The positive rate of E-cadherin in the breast cancer was 70.0%(63/90),which was lower than that in breast fibroadenoma[95.0%(38/40);χ^(2)=9.998,P<0.001].The positive rate of Vimentin in the breast cancer was 34.4%(31/90),which was higher than that in the breast fibroadenoma[0.0%(0/40);χ^(2)=18.091,P<0.001].The positive rate of VEGF-C in the breast cancer was 46.7%(42/90),which was higher than that in breast fibroadenoma[0.0%(0/40);χ^(2)=27.582,P<0.001].The positive rate of MVD in the breast cancer was 96.7%(87/90),which was higher than that in the breast fibroadenoma[0.0%(0/40);χ^(2)=116.902,P<0.001].HIF-1αexpression was correlated with histological grade and TNM stage(χ^(2)=8.901,P=0.012;χ^(2)=8.711,P=0.013).E-cadherin expression was correlated with tumor size and histological grade(χ^(2)=5.981,P=0.050;χ^(2)=6.051,P=0.048).Vimentin expression was correlated with tumor size,lymph node metastasis and TNM stage(χ^(2)=6.047,P=0.049;χ^(2)=26.683,P<0.001;χ^(2)=28.651,P<0.001).VEGF-C expression was associated with lymph node metastasis,TNM stage and molecular typing(χ^(2)=12.562,P<0.001;χ^(2)=17.803,P<0.001;χ^(2)=13.485,P=0.004).MVD was associated with tumor size(χ^(2)=8.595,P=0.014).HIF-1αwas negatively correlated with E-cadherin expression(r=-0.513,P<0.001),and was positively correlated wi
作者
曹卫刚
杨振林
CAO Weigang;YANG Zhenlin(Department of Breast Surgery,Yantai Affiliated Hospital of Binzhou Medical University,Yantai 264100,China)
出处
《肿瘤基础与临床》
2023年第4期277-281,共5页
journal of basic and clinical oncology
基金
山东省自然科学基金资助项目(ZR2017MH033)
烟台市重点研发计划项目(2019YD063)
滨州医学院校级科技计划项目(BY2018KJ31)。
