摘要
血管生成素4(ANG4)是一种新型抗菌肽蛋白,具有抗菌、抗炎、血管生成、调节机体免疫反应等多种生物学功能,在机体先天免疫过程中发挥重要的作用。ANG4在新生小鼠肠道的表达受到多种因素的调节,如细菌信号和发育过程等。本试验选取细菌细胞壁表面分子的类似物壳寡糖(COS)作为诱导物,探究其对小鼠肠道ANG4表达和对肠道形态的影响。实时荧光定量聚合酶链式反应(qRT-PCR)和蛋白质免疫印迹(Western blot)分析表明,COS能增加小鼠肠道ANG4 mRNA及ANG4蛋白的表达;形态学观察结果显示,COS使小肠绒毛高度明显增加,平均隐窝深度均有降低,V/C值增加,表明COS可通过诱导ANG4等抗菌蛋白的表达,或通过其杀菌和免疫调节作用而不同程度地提高小肠黏膜的完整性。研究结果为COS以及ANG4在仔猪肠道疾病防治的应用提供了理论依据。
Angiogenin 4(ANG4),a novel antimicrobial protein with multiple biological functions,such as antimicrobial,anti-inflammation,angiogenesis and regulating the body’s immune response,plays a key role in innate immunity.The expression of ANG4 in neonatal mice intestine is regulated by some factors including microbial signal and developmental stage.Being an analogue of surface molecules of bacterial cell wall,chitosan oligosaccharide(COS)was selected as a inducer to explore the effects on ANG4 expression and mophology in post weaning mice intestine.qRT-PCR and Western blot analyses showed that the mRNA and protein levels of ANG4 increased suggesting that COS can upregulate the expression of ANG4.The results of HE staining of mouse intestinal tract showed that the height of intestinal villi,the depth of crypt and the value of V/C increased significantly.It indicates that COS can improve the integrity of intestinal mucosae in some extent through either inducing the expression of some intestinal antimicrobial proteins,such as ANG4,or its antimicrobial and immunomodulation activities,and provide the theoretical basis for the applications of COS and ANG4 in controlling the intestinal diseases in piglets.
作者
贺宇文
汤冠宇
李楚楚
李灿
纪春晓
陈韬
HE Yuwen;TANG Guanyu;LI Chuchu;LI Can;JI Chunxiao;CHEN Tao(College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China;Hunan Provincial Institution of Veterinary Drug and Feed Control,Changsha 410006,China;Hunan Veterinary Drug Engineering and Technology Research Center,Changsha 410128,China)
出处
《激光生物学报》
CAS
2023年第3期234-239,共6页
Acta Laser Biology Sinica
基金
国家自然科学基金项目(31772633)
湖南农业大学“双一流”建设项目(SYL2019050)。
