摘要
用凹凸棒黏土和硝酸铜溶液采用离子交换的方法制备成载铜凹凸棒黏土,将载铜凹凸棒黏土添加于饲料中研究其对鸡白痢的预防和治疗效果。预防试验:将100只1日龄AA蛋鸡随机分成5组,空白组,感染组,预防1组,预防2组,氯霉素预防组。空白组不做任何处理,饲喂正常饲料;感染组于1~15日龄饲喂正常饲料,7日龄灌胃鸡白痢沙门菌菌液;预防组1~15日龄饲喂不同含量的载铜凹凸棒黏土和氯霉素作为预防性给药,7日龄灌胃鸡白痢沙门菌菌液。治疗试验:将100只1日龄鸡随机分成5组,空白组,感染组,治疗1组,治疗2组,氯霉素治疗组。空白组不做任何处理,饲喂正常饲料;感染组于7日龄灌胃鸡白痢沙门菌菌液,饲喂正常饲料;治疗组7日龄灌胃鸡白痢沙门菌菌液,饲喂不同含量的载铜凹凸棒粘土和氯霉素作为治疗性给药。试验过程中监测记录发病率、死亡率、保护率等,平板法测定盲肠和肝脏带菌情况,称重法测定生产性能,常规切片观察空肠组织形态。预防试验结果显示:添加1%的载铜凹凸棒黏土组鸡白痢的发病率(20%)、死亡率(10%)降低,肝脏和盲肠沙门菌的检出率降低,鸡的生产性能提高,肠道黏膜完整,且效果好于添加0.05%氯霉素组和添加0.5%凹凸棒黏土组。治疗试验结果显示:添加1.5%的载铜凹凸棒黏土组鸡白痢的治愈率(75%)增加,死亡率(10%)降低,肝脏和盲肠沙门菌的检出率降低,鸡的生产性能提高,效果好于添加0.1%氯霉素组和添加1%凹凸棒黏土组。结果表明:载铜凹凸棒黏土具有抗菌和肠道黏膜保护作用,能够预防和治疗鸡白痢,降低鸡白痢沙门菌对机体的损伤,提高鸡的生产性能。
Copper-modified attapulgite clay was made of attapulgite clay and copper nitrate solution by ion exchange. The effects of copper-modified attapulgite clay on prevention and treatment of pullorum diseases were studied by mixing it with feed. In the preventive test,one hundred one day- old chickens were randomly divided into five groups, control group, infection group, No. 1 preven- tion group,No. 2 prevention group and chloramphenicol prevention group. Control group was fed normal diet without any treatment. Infection group was fed normal diet from 1st day to 15th day and infected with Salmonella pullorum at the 7th day. Prevention groups were given different lev- els of copper-modified attapulgite clay or chloramphenicol as prophylactic administration from 1st day to 15th day. Prevention groups were also infected with Salmonella pullorum at the 7th day. In the treatment test, one hundred one-day-old chickens were randomly divided into five groups, con- trol group, infection group, No. 1 treatment group, No. 2 treatment group and chloramphenicol treatment group. Control group was fed normal diet without any treatment. Infection group was given normal diet and infected with salmonella pullorurn at the 7th day. Treatment groups were infected with Salmonella pullorum at the 7th day and then given different levels of copper-modi- fied attapulgite clay or chloramphenicol as treatment administration. During the test, morbidity, mortality,protection rate were monitored and recorded. Salmonella infection of cecum and liver were determined by SS plate culture. The weights of chicken and feed intake were recorded for measuring the growth performance. The morphology of jejunal was examined using optical micro scope by histopathology. The results of prevention test showed that morbidity, mortality and isola- ting rate of Salmonella in live and caecum were decreased when adding copper-modified attapulgite clay in feed （1 %）. The growth performance of chickens was improved. The effect of 1% copper modified attapulgite clay
作者
李娜
姚大伟
许家荣
杨芸
杨德吉
LI Na YAO Da-wei XU Jia-rong YANG Yun YANG De-ji(College of Veterinary Medicine, Nanjing Agricultural University , Nanjing 210095 ,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2017年第7期1365-1371,共7页
Chinese Journal of Veterinary Science
基金
江苏省凹土资源利用重点实验室开放研究基金资助项目(HPK201506)
通过产学研活动培养应用型创新人才和研究型人才的探索与实践资助项目(2013Y021)
关键词
载铜凹凸棒黏土
鸡白痢
沙门菌
copper-modified attapulgite clay
pulloram diseases
Salmonella pullorum
