摘要
目的:探讨人乳头瘤病毒(HPV)分型定量检测在宫颈癌筛查中的临床价值。方法:收集2019年5月-2021年5月本院收治并有细胞学和(或)组织病理学结果的204例HPV阳性患者资料,采用分型定量检测试剂盒(BMRT)检测21种HPV病毒并进行分型和标准化定量,比较不同HPV亚型病毒载量与宫颈病变程度关系,分析预测≥HSIL的HPV亚型病毒载量最佳临界值。结果:高危HPV感染率前3位为HPV16(45.6%)、HPV18(14.5%)、HPV52(5.4%)。不同细胞学分级者HPV16、18、58、68亚型病毒载量有差异且与分级成正相关,不同病理学分期者HPV16、52、58和68亚型病毒载量有差异且与分期成正相关(均P<0.05)。当HPV16、52、58亚型病毒载量临界值达到4.18、3.478和3.875时,可预测发生宫颈病变≥HSIL的风险,曲线下面积分别为0.839、0.738、0.837。结论:HPV亚型病毒载量与宫颈病变的严重程度相关,BMRT HPV检测对宫颈病变初筛有一定意义。
Objective:To explore the clinical value of quantitative detection of human papillomavirus(HPV)typing for screening cervical cancer.Methods:The clinical data of 204patients with HPV positive and cytology and/or histopathology from May 2019to May 2021were collected retrospectively.21kinds of HPV were typed and quantified by Bio-Perfectus Multiplex Real Time(BMRT)detection kit.The correlation between the viral load of different subtype HPV and the degree of cervical lesions was compared.The optimal cut off value of the viral load of subtype HPV for predicting≥HSIL of cervical lesions was analyzed.Results:The top three high infection rates of subtype HPV were HPV 16(45.6%),HPV 18(14.5%),and HPV 52(5.4%).In different cytological grades,the viral load of subtypes HPV16,18,58,and 68had significant differences and were positively correlated with the grades.In different pathological grades,the viral load of subtypes HPV 16,52,58and 68had significant differences and were positively correlated with the grades of cervical lesions(all P<0.05).When the cut off values of subtypes HPV16,52,and 58reached 4.18,3.478,and 3.875,the area under the curve(AUC)of subtypes HPV16,52,and 58for predicting the risk of cervical lesions≥HSIL were 0.839,0.738,and 0.837,respectively.Conclusion:The viral loads of subtypes HPV are significantly related to the severity of cervical lesions.The detections of HPV by BMRT have certain significance in the primary screening of cervical lesion.
作者
赵永秀
胡杨
季俊
林蔚
李梅
丁邦宇
周青
吴玉璘
ZHAO Yongxiu;HU Yang;JI Jun;LIN Wei;LI Mei;DING Bangyu;ZHOU Qing;WU Yulin(Taixing Maternity and Child Health Care Hospital,Taixing,Jiangsu Province,225400;Jiangsu Health DevelopmentResearch Center,National Health Commission Contraceptives Adverse Reaction Surveillance Center,Nanjing)
出处
《中国计划生育学杂志》
2023年第6期1478-1482,共5页
Chinese Journal of Family Planning
基金
江苏省科技厅创新能力建设计划(BM2018033-3)。
关键词
宫颈病变筛查
宫颈上皮内瘤变
人乳头瘤病毒
病毒载量
预测价值
Cervical lesions
Cervical intraepithelial neoplasia
Human papillomavirus
Viral load
Predictive value
