摘要
为分析紫花含笑不同开花时期花被片挥发性成分,确定其主要花挥发性物质,以花蕾期、初花期和盛花期的花被片为试验材料,采用HS–SPME–GC–Orbitrap–HRMS法对其挥发性成分进行检测。从紫花含笑花被片中共鉴定出641种挥发物,共14类化合物,以萜类和酯类物质为主;盛花期花被片挥发物种类最多(434种),初花期次之(392种),花蕾期最少(352种),各时期分别检测到特有挥发物96、80和118种,特别是在盛花期,检测到了3种特有生物碱类:四氢东非马钱碱、托品酮和毛果芸香碱;盛花期花被片挥发物总含量最高(668.993μg·kg^(-1)),初花期次之(628.111μg·kg^(-1)),花蕾期最低(522.590μg·kg^(-1));含量较高(>13.000μg·kg^(-1))的主要挥发物有α–愈创木烯、大根香叶烯B、顺–衣兰油–4(15),5–二烯、β–石竹烯、双环倍半水芹烯、顺式–β–罗勒烯、β–律草烯、双环大根香叶烯、γ–依兰油烯和α–古巴烯;香气强度(Odor activity value,OAV)分析表明:紫花含笑花被片特征香气成分包括苯甲酸甲酯和α–蒎烯,修饰性香气成分包括顺式–β–罗勒烯、β–石竹烯、芳樟醇和橙花叔醇,使其香气特征呈香草型、花香型和木香型;低香气阈值的β–紫罗兰酮使初花期花被片较花蕾期呈明显花香型;乙酸苄酯是盛花期花被片特有挥发物,使其呈浓甜香型。主成分分析结果表明:紫花含笑花被片香气特征不是被高含量挥发物主导,而是被多种挥发物相互作用且相互影响。综上表明,紫花含笑花被片的挥发性成分组成丰富,但不同花期间存在一定差异;挥发性成分的种类、含量和香气阈值的差异影响了不同花期花被片的香气特征,使其呈现出多样的花香。
The volatile components of Michelia crassipes tepals at the flower bud stage,the first-open stage and fully-bloom stage were analyzed using by HS–SPME–GC–Orbitrap–HRMS.A total of 641volatiles,which could be further classified into 14 groups(mainly terpenes and esters),were identified.Among them,434 volatiles were found in the tepals at the fully-bloom stage,392 volatiles at the first-open stage,and 352 volatiles at the flower bud stage.Also 96,80 and 118 unique volatiles were detected in each stage.At the fully-bloom stage,3 unique alkaloids were detected and they were tetrahydrologanine,tropinone and pilocarpine.The total amount of volatile compounds at fully-open stage was the highest of 668.993μg·kg^(-1),followed by the first-open stage at 628.111μg·kg^(-1),and the lowest at the bud stage of 522.590μg·kg^(-1).α-guaiene,germacrene B,cis-muurola-4(15),5-diene,β-caryophyllene,bicyclosesquiphellandrene,cis-β-ocimene,β-humulene,bicyclogermacrene,γ-muurolene andα–copaene were the major volatiles(content over 13.000μg·kg^(-1)).The analysis of OAV concluded that the characteristic aroma components of M.crassipes tepals included methyl benzoate andα-pinene and the modified aroma components included cis-β-ocimene,β-caryophyllene,linalool and nerolidol.Herbal,floral and woody odor types were the main aroma characteristics.Β-ionone with a low aroma threshold made the tepals in the first-open stage much more floral odor type than that in the bud stage.Benzyl acetate was an unique volatile compound of tepals in fully-open stage,which contributed its strong sweet odor type.The results of principal component analysis indicated that the aroma characteristics of tepals of M.crassipes were not dominated by high-content volatiles,but by the interaction and mutual influence of multiple volatiles.In conclusion,the composition of volatile components in the tepals of M.crassipes is rich,but there are certain differences in different flowering stages.The differences in the types,contents and aroma threshold
作者
袁婕俐
金晓玲
张峥
肖玉洁
余秋岫
胡筱璇
YUAN Jieli;JIN Xiaoling;ZHANG Zheng;XIAO Yujie;YU Quxiu;HU Xiaoxuan(College of Landscape Architecture,Central South University of Forestry and Technology,Changsha 410004,China;Hunan Big Data Engineering Technology Research Center of Natural Protected Areas Landscape Resources,Changsha 410004,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2023年第5期1095-1109,共15页
Acta Horticulturae Sinica
基金
国家林业局重点学科项目(林人发[2016]21号)
湖南省“双一流”培育学科项目(湘教通[2018]469号)
2021年湖南省研究生科研创新项目(CX20210870)
中南林业科技大学2021年研究生科技创新基金项目(CX202102046)。
