摘要
中药复杂体系中药效物质的识别及与作用靶标相互作用的表征确证,是困扰中药现代化研究的瓶颈难题。近年来,多种分子互作表征技术被成功应用于中药研究领域,用来识别和表征中药的关键药效物质与疾病作用靶点的分子间相互作用。这些研究结果为探索疾病发生发展的病理基础、诠释中药关键药效物质与其作用靶点精准互作的药效机制提供理论依据。通过对表面等离子体共振、等温滴定量热、生物膜干涉和微量热泳动4种主要的分子互作表征技术的原理、优势特点与应用进展进行综述,为中药现代化研究提供方法策略的参考依据。
The identification of active substances in complex system of traditional Chinese medicine(TCM)and the characterization and confirmation of the interaction with the targets are the bottleneck problems plaguing the modernization research of TCM.In recent years,a variety of molecular interaction characterization techniques have emerged and been successfully applied to identify and characterize the intermolecular interactions between key active substances and their disease targets in field of TCM research.These research results will provide a theoretical basis for exploring the pathological basis of the occurrence and development of the disease and interpreting the pharmacodynamic mechanism of accurate interaction between the key pharmacodynamic substances of TCM and their action targets.The principles,advantages,and application advances of four major molecular interaction characterization techniques,such as surface plasmon resonance,isothermal titration calorimetry,bio layer interferometry and microscale thermophoresis,were reviewed,to provide reference for methodological strategies in research of TCM modernization.
作者
袁露萍
方文秀
李梦颖
吴优
许晓莹
秦路平
王小艳
YUAN Lu-ping;FANG Wen-xiu;LI Meng-ying;WU You;Xu Xiao-ying;QIN Lu-ping;WANG Xiao-yan(Zhejiang Chinese Medical University,Hangzhou 310000,China)
出处
《中草药》
CAS
CSCD
北大核心
2023年第10期3370-3377,共8页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(81973571)
浙江中医药大学远志优青(2019)。
关键词
中药药效物质
药物靶点
分子间相互作用
表面等离子体共振
等温滴定量热
生物膜干涉
微量热泳动
traditional Chinese medicinal active substance
drug target
intermolecular interaction
surface plasmon resonance
isothermal titration calorimetry
bio layer interferometry
microscale thermophoresis
