摘要
目的探讨四个半LIM结构域蛋白2(FHL2)与前B细胞白血病同源盒基因3(PBX3)在卵巢癌(OC)组织中的表达与临床病理特征及预后的相关性。方法选取广东省阳江市人民医院2017年6月至2019年6月收治的63例OC患者作为研究对象,收集患者的OC组织和癌旁正常组织(距肿瘤边缘4 cm处)进行研究,采用免疫组化法检测组织中的FHL2与PBX3蛋白的表达水平,并分析其与OC患者临床病理特征的关系,采用Cox回归模型分析影响OC患者预后的相关因素。结果患者癌组织中FHL2和PBX3蛋白表达阳性率显著高于癌旁组织(P<0.05);FHL2与PBX3蛋白表达与肿瘤分化程度、淋巴结转移、腹水和病理分期具有显著相关性(P<0.05);所有OC患者中位生存期为30.75(24.82,36.70)个月,其中FHL2阳性和阴性表达OC患者的中位生存期分别为17.67(6.33,29.01)和35.36(32.30,38.41)个月,PBX3阳性和阴性表达OC患者的中位生存期分别为17.01(14.11,19.91)和33.75(31.45,36.05)个月,差异具有统计学意义(Log rank P<0.05)。经单因素分析,肿瘤低分化、淋巴结转移、病理分期Ⅲ+Ⅳ期、FHL2及PBX3阳性表达患者的生存时间均显著缩短,差异具有统计学意义(P<0.05);Cox回归模型分析表明,淋巴转移情况(HR=0.17,95%CI:0.05~0.53)、腹水(HR=0.02,95%CI:0.00~0.17)、病理分期(HR=11.45,95%CI:2.95~44.49)、FHL2(HR=0.19,95%CI:0.05~0.71)及PBX3表达水平(HR=0.22,95%CI:0.08~0.57)均为OC患者不良预后的独立危险因素(P<0.05)。结论FHL2与PBX3蛋白在OC组织中的表达水平显著高于癌旁正常组织,且表达水平与OC患者的临床特征显著相关。该研究对于OC的发生、发展及预后均有着重要的临床意义。
Objective To investigate the correlation between the expression of four and a half LIM domains 2(FHL2)and pre-B-cell leukemia transcription factor 3(PBX3)in ovarian cancer(OC)tissues and the clinicopathological features and prognosis.Methods A total of 63 OC patients admitted to People’s Hospital of Yangjiang from June 2017 to June 2019 were selected as research subjects.The OC tissues and paracancerous normal tissues(4 cm from the tumor margin)of the patients were collected for research.The expression levels of FHL2 and PBX3 proteins in tissues were detected by immunohistochemical method,and their relationship with clinicopathological features of OC patients was analyzed.Cox regression model was used to analyze the relevant factors affecting the prognosis of OC patients.Results The positive rate of FHL2 and PBX3 protein expression in cancer tissues of OC patients was significantly higher than that in paracancerous tissues(P<0.05);FHL2 and PBX3 protein expression was significantly correlated with tumor differentiation,lymph node metastasis,ascites and pathological stage(P<0.05).The median survival time of all OC patients was 30.75(24.82,36.70)months,among which the median survival time of OC patients with FHL2 positive and negative was 17.67(6.33,29.01)and 35.36(32.30,38.41)months,respectively,the median survival time of OC patients with PBX3 positive and negative was 17.01(14.11,19.91)and 33.75(31.45,36.05)months,respectively,and the difference was statistically significant(Log rank P<0.05).Univariate analysis showed that the survival time of patients with poorly differentiated tumors,lymph node metastasis,pathological stage III+IV,FHL2 and PBX3 positive expression was significantly shortened,and the difference was statistically significant(P<0.05).Cox regression model analysis showed that lymphatic metastasis(HR=0.17,95%CI:0.05-0.53),ascites(HR=0.02,95%CI:0.00-0.17),pathological stage(HR=11.45,95%CI:2.95-44.49),and expression levels of FHL2(HR=0.19,95%CI:0.05-0.71)and PBX3(HR=0.22,95%CI:0.08-0.57)were indepen
作者
黄小杏
梁秀冰
莫劲思
黄世勇
郝丽莉
李江
曾庆苏
HUANG Xiaoxing;LIANG Xiubing;MO Jinsi;HUANG Shiyong;HAO Lili;LI Jiang;ZENG Qingsu(Department of Pathology,People’s Hospital of Yangjiang,Yangjiang 529500,China)
出处
《空军军医大学学报》
CAS
2023年第5期453-457,共5页
Journal of Air Force Medical University
基金
广东省基础与应用基础研究基金(2020B1515120231)
广东省阳江市医疗卫生科技计划项目(SF2021042)。
