摘要
目的探讨前B细胞白血病同源盒基因3(PBX3)在急性髓系白血病(AML)患者骨髓样本中的表达及其临床意义。方法通过生物信息学方法,分析美国癌症和肿瘤基因图谱计划数据库(TCGA)33种类型癌症转录组测序数据,获得PBX3 mRNA在泛癌中的表达水平,进一步分析AML患者中PBX3 mRNA表达水平与患者临床病理特征、预后的关系。利用全转录组基因表达差异分析,探究AML中PBX3相关分子特征。结果PBX3 mRNA表达水平在12种癌症类型中上调,其中在AML患者中上调最为显著。PBX3高表达AML患者总生存时间和无病生存时间较PBX3低表达患者缩短,且与AML分子遗传学事件FLT3、NPM1和DNMT3A基因突变有关。PBX3表达与多个同源盒基因(包括多数HOXA和HOXB基因、MEIS1)呈正相关,且这些同源盒基因在AML患者中表达水平均与总生存时间呈负相关。结论AML患者骨髓中PBX3基因高表达可能是预后不良的潜在生物标志物,其与同源盒基因可能存在广泛的相互作用。
Objective To investigate the expression levels and potential clinical significance of pre-B-cell leukemia homologous box 3(PBX3)in bone marrow samples from acute myeloid leukemia(AML).Methods The mRNA expression levels of PBX3 were determined by bioinformatics which analyzed the RNAseq data of 33 malignancies from the cancer genome atlasdatabase.(TCGA)The correlations among the expression levels of PBX3,the clinical parameters and prognosis of AML patients were further analyzed.The differentially expressed genes in the whole transcriptome were analyzed to identify the molecular network in AML caused by PBX3 expression abnormalities.Results The mRNA expression levels of PBX3 were up-regulated in 12 malignancies,and the altitudes increased most significantly in AML than any other cancer types.Patients with high PBX3 expression showed shorter overall survival and disease-free survival than patients with low PBX3 expression.High PBX3 expression was significantly associated with FLT3,NPM1,and DNMT3A mutation.PBX3 expression was positively correlated with multiple homeobox genes(including most HOXA and HOXB genes,MEIS1),and the expression levels of these homeobox genes were all negatively correlated with AML patients overall survival.Conclusion PBX3 high expression in the bone marrow of AML patients is a potential biomarker for poor prognosis,and it may have extensive interactions with other homeobox genes.
作者
张慧
陈子翔
赵纯毅
邵启祥
赵杨静
Zhang Hui;Chen Zixiang;Zhao Chunyi;Shao Qixiang;Zhao Yangjing(Dept of Clinical Laboratory,The Affiliated Hospital of Jiangsu University,Zhenjiang 212001;Dept of Laboratory Medicine,School of Medicine,Jiangsu University,Zhenjiang 212013)
出处
《安徽医科大学学报》
CAS
北大核心
2022年第12期2012-2018,2024,共8页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:82100183,81701545)
江苏省大学生创新创业训练计划项目(编号:2021102991051X)。
