摘要
[目的]探讨腹泻型肠易激综合征(diarrhea-predominant irritable bowel syndrome,IBS-D)大鼠肠道菌群组成与血清炎症因子变化及两者相关性。[方法]选取12只雄性SD大鼠按随机数字表法分为正常组与模型组,每组6只。模型组依照“乙酸结肠灌注联合束缚刺激法”构建IBS-D大鼠模型。正常组、模型组大鼠均通过灌胃给予蒸馏水10 mL/(kg·BW),1次/d,连续干预8 d后进行样本采集。应用ELISA法检测血清中IL-10、IL-6、TNF-α含量,并利用16S rDNA测序结果对2组大鼠肠道菌群物种组成、单因素网络以及与血清炎症因子的相关性等进行分析。[结果]与正常组相比,模型组IL-10水平降低(P<0.01),而IL-6、TNF-α水平提高(P<0.01)。物种组成比较显示,2组肠道菌群在门和属水平上存在差异,与正常组相比,在门水平上,模型组拟杆菌门(Bacteroidota)相对丰度增加,厚壁菌门(Firmicutes)等相对丰度降低(P<0.05或P<0.01);在属水平上,模型组拟普雷沃菌属(Alloprevotella)等相对丰度增加,Lachnospiraceae_NK4A136_group等相对丰度降低(P<0.05)。肠道菌群指示物种分析显示,模型组Candidatus_Stoquefichus、Candidatus_Arthromitus等具有显著效应(P<0.05)。单因素网络分析结果显示,模型组的差异菌群Alloprevotella与罗氏菌属(Roseburia)、罗姆布茨菌属(Romboutsia)、Lachnospiraceae_NK4A136_group呈负相关,而Lachnospiraceae_NK4A136_group与Romboutsia、UCG-003、Oscillibacter、Roseburia呈正相关。相关性分析结果显示,门水平上,梭杆菌门(Fusobacteriota)、黏菌门(Myxococcota)、变形菌门(Proteobacteria)、盐厌氧菌门(Halanaerobiaeota)与IL-10水平呈正相关,Bacteroidota与IL-10水平呈负相关,Bacteroidota与IL-6水平呈正相关,Campilobacterota、Halanaerobiaeota与IL-6水平呈负相关,脱硫菌门(Desulfobacterota)、Campilobacterota、Halanaerobiaeota与TNF-α呈负相关;在属水平上,Alloprevotella、Prevotellaceae_UCG-001、普雷沃菌属(Prevotella)与IL-10呈正
[Objective]This study was conducted to characterize the changes in intestinal microbiota composition and serum inflammatory factors as well as their correlation in rats with diarrhea-predominant irritable bowel syndrome(IBS-D).[Method]A total of 12 male SD rats were divided into a normal group and a model group according to the random number table,with 6 rats in each group.The IBS-D rat models were established using acetic acid colon perfusion combined with restraint stimulation method.Normal and model rats were given 10 mL/(kg·BW)of distilled water by gavage once per day.Samples were collected after 8 consecutive days of intervention.ELISA assay was used to measure the serum levels of IL-10,IL-6 and TNF-α.The species composition of intestinal microbiota,single factor network,and correlation of intestinal microbiota with serum inflammatory factors in the two groups of rats were examined using the 16S rDNA sequencing data.[Result]In comparison with the normal group,the serum level of IL-10 in the model group decreased(P<0.01),while the serum levels of IL-6 and TNF-αincreased(P<0.01).The comparison of species composition showed that there were differences in intestinal microbiota at the phylum and genus levels between the two groups.Compared with the normal group,at the phylum level,the relative abundance of Bacteroidota in the model group increased,while that of Firmicutes and other bacteria decreased(P<0.05 or P<0.01);at the genus level,the relative abundance of Alloprevotella in the model group elevated,while that of Lachnospiraceae_NK4A136_group and other bacteria reduced(P<0.05).Analysis of indicator species of intestinal microbiota showed that Candidatus_Stoquefichus,Candidatus_Arthromitus and other bacteria in the model group had significant(P<0.05)affects.The results of single factor network analysis showed that in the model group,the differential microbiota Alloprevotella was negatively correlated with Roseburia,Romboutsia and Lachnospiraceae_NK4A136_group,while Lachnospiraceae_NK4A136_group was positiv
作者
张儒奇
朱炳旭
孙金鹏
王荣
孙闵
ZHANG Ruqi;ZHU Bingxu;SUN Jinpeng;WANG Rong;SUN Min(College of Integrated Traditional Chinese and Western Medicine,Jining Medical University,Jining 272067,China;College of Basic Medicine,Jining Medical University,Jining 272067,China)
出处
《畜牧与饲料科学》
2023年第2期17-25,共9页
Animal Husbandry and Feed Science
基金
山东省中医药重点学科建设项目(鲁卫中医药科教字[2022]4号)
济宁医学院中西医结合治疗脾胃病创新团队项目(jy201918)。
关键词
腹泻型肠易激综合征
肠道菌群
血清炎症因子
相关性
diarrhea-predominant irritable bowel syndrome
intestinal microbiota
serum inflammatory factor
correlation
