摘要
目的:通过观察针刺对应激性胃溃疡(SGU)大鼠血清中炎性因子及肠道菌群的影响,探讨针刺治疗SGU的机制。方法:将SD大鼠随机分为空白组、模型组、针刺组、药物组,每组7只。采用改良的束缚-浸水应激法制备SGU模型。针刺组针刺"中脘"及双侧"足三里",留针20 min,每日1次,连续5 d。药物组给予奥美拉唑肠溶片溶液2 mL灌胃(0.2 mg/kg),每日1次,连续5 d。用Guth法计算胃黏膜损伤指数,HE染色法观察大鼠胃黏膜组织形态学改变,用ELISA法检测血清中白细胞介素(IL)-4、IL-6的含量,用16S rDNA测序法检测肠道菌群变化。结果:造模后,与空白组相比,模型组大鼠胃黏膜损伤指数明显升高(P<0.01),HE染色显示大鼠胃黏膜损伤严重,血清IL-4含量明显降低(P<0.01)、IL-6含量明显升高(P<0.01),肠道菌群微生物中,拟杆菌门、厚壁菌门丰度均降低,变形菌门、放线菌门和脱铁杆菌门丰度升高。与模型组相比,针刺组和药物组大鼠胃黏膜损伤指数明显降低(P<0.01,P<0.05),HE染色显示大鼠胃黏膜病理变化有所改善,大鼠血清IL-4含量明显升高(P<0.01,P<0.05)、IL-6含量明显降低(P<0.01,P<0.05),拟杆菌门丰度升高。与药物组相比,针刺组胃黏膜损伤指数降低(P<0.05),血清IL-4含量明显升高(P<0.05)、IL-6含量明显降低(P<0.05)。结论:针刺"中脘""足三里"可通过上调血清IL-4水平、下调IL-6水平,部分调控肠道微生物菌群结构,达到修复胃黏膜损伤的治疗效果。
Objective To investigate the effect of acupuncture on serum inflammatory cytokines and intestinal flora in rats with stress-induced gastric ulcer(SGU),and to explore the mechanism of acupuncture in the treatment of SGU.Methods Sprague-Dawley rats were randomly divided into blank,model,acupuncture,and medication groups,with 7 rats in each group.Restraint water-immersion stress was used to establish the model of SGU.The rats in the acupuncture group were given acupuncture at"Zhongwan"(CV12)and bilateral"Zusanli"(ST36)for 20 min,once a day for 5 days,and those in the medication group were given 2 mL solution of Omeprazole enteric-coated tablets(0.2 mg/kg)by gavage,once a day for 5 days.The Guth method was used to calculate the gastric mucosa damage index,HE staining was used to observe the histopathological changes of the gastric mucosa,ELISA was used to measure the serum levels of interleukin-4(IL-4)and interleukin-6(IL-6),and 16 S rDNA sequencing method was used to observe the change in intestinal flora.Results Compared with the blank group,the model group had a significant increase in gastric mucosa damage index(P<0.01),markedly pathological changes of the gastric mucosa shown by HE staining,a significant reduction in the content of serum IL-4(P<0.01),and a significant increase in the content of serum IL-6(P<0.01),as well as a significant reduction in Bacteroidetes and Firmicutes at the phylum level.Compared with the model group,the acupuncture group and the medication group had a significant reduction in gastric mucosa damage index(P<0.01,P<0.05).HE staining showed reduced pathological changes of the gastric mucosa,as well as a significant increase in the content of serum IL-4(P<0.01,P<0.05)and a significant reduction in the content of serum IL-6(P<0.01,P<0.05).As for the intestinal flora,there was a significant increase in Bacteroidetes.Compared with the medication group,the gastric mucosa damage index was decreased(P<0.05),the content of serum IL-4 was significantly increased(P<0.05),while the content of serum
作者
薛婷
王旒靖
吴颖琦
王图南
赵珈宇
李婧婷
马佳佳
付晨露
张鹏
邵一轩
杨易陈
周子娴
马惠芳
XUE Ting;WANG Liu-jing;WU Ying-qi;WANG Tu-nan;ZHAO Jia-yu;LI Jing-ting;MA Jia-jia;FU Chen-lu;ZHANG Peng;SHAO Yi-xuan;YANG Yi-chen;ZHOU Zi-xian;MA Hui-fang(School of Acupuncture-Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 100029,China;Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700;Beijing Airport Hospital,Beijing 101300;Community Health Service Center,Hongshunli street,Hebei District,Tianjin 300142;Wangjing Community Health Service Center,Chaoyang District,Beijing 100102)
出处
《针刺研究》
CAS
CSCD
北大核心
2020年第5期379-383,共5页
Acupuncture Research
关键词
应激性胃溃疡
针刺
白细胞介素
肠道菌群
Stress-induced gastric ulcer
Acupuncture
Interleukin
Intestinal flora
