摘要
建立了差示扫描量热法测定辣椒素药物纯度的方法。考察了不同升温速率和辣椒素样品质量对测定结果的影响,确定了差示扫描量热法最佳条件为:升温速率2 K·min^(-1),辣椒素样品质量为1.5~3.5 mg。该法与高效液相色谱、气相色谱-质谱以及核磁共振氢谱等方法的测定结果进行了比较,结果表明:差示扫描量热法、高效液相色谱法、气相色谱/质谱法和核磁共振氢谱法测定的辣椒素质量分数分别为98.88%、98.88%、98.04%和98.97%,最大偏差为0.93%。讨论了产生偏差的原因。差示扫描量热法操作简单、结果准确,可作为测定辣椒素药物纯度的方法。
A differential scanning calorimetry single-peak method was established to determine the purity of synthetic capsaicin drugs.By investigating the influence of different heating rates and sample mass on the determination results,the best experimental conditions of differential scanning calorimetry single peak method were determined as the heating rate is controlled at 2 K·min^(-1),and the sample mass is controlled at 1.5~3.5 mg.These results were compared to the measurement results of high performance liquid chromatography,gas chromatography-mass spectrometry and proton nuclear magnetic resonance spectroscopy.The results showed that the purities determined by differential scanning calorimetry single peak method,high performance liquid chromatography,gas chromatography/mass spectrometry and proton nuclear magnetic resonance spectroscopy were 98.88%,98.88%,98.04%and 98.97%,respectively,and the maximum deviation was O.93%.The reasons for the deviation were discussed to obtain the conclusion that the differential scanning calorimetry unimodal method is simple and accurate,and can be used as a supplementary method for the determination of the purity of synthetic capsaicin drugs.
作者
段晶钟
郝尚鹏
黄桂兰
高润利
张远鹏
汪海涛
Duan Jingzhong;Hao Shangpeng;Huang Guilan;Gao Runli;Zhang Yuanpeng;Wang Haitao(State Key Laboratory of Nuclear Bio Chemistry Protection for Civilian,Beijing 102205,China;School of Environment and Safety Engineering,North University of China,Taiyuan,Shanzi 038507,China)
出处
《化学世界》
CAS
2023年第2期110-115,共6页
Chemical World
基金
国家自然科学基金面上(No.21876202)资助项目。
关键词
辣椒素
差示扫描量热法
高效液相色谱法
capsaicin
differential scanning calorimetry
high performance liquid chromatography
