摘要
目的研究宫颈癌患者外周血免疫细胞群及癌组织内泛素蛋白1(UBQLN1)表达水平与其预后的关系。方法将2018年6月至2020年1月在晋城大医院行宫颈癌根治术联合术后规范化治疗的98例宫颈癌患者纳为观察组,同时将同期于同一医院行健康体检的60例同年龄段女性纳为对照组。术前采集2组研究对象外周静脉血,检测CD4^(+)T、CD8^(+)T细胞以及CD28^(+)T细胞表达水平,并于术中收集观察组患者宫颈癌及癌旁组织,采用免疫组化法检测UBQLN1表达水平。随访至2022年1月,统计观察组患者宫颈癌复发率。分析外周血免疫细胞群及宫颈癌组织内BUQLN1表达水平与其临床病理特征及预后之间的关系。结果观察组外周血CD8^(+)T细胞占比高于对照组,CD4^(+)/CD8^(+)比值低于对照组,观察组宫颈癌组织内UBUQLN1阳性率高于癌旁组织,差异均有统计学意义(P<0.05)。宫颈癌临床分期为ⅡA期的患者外周血CD4^(+)T细胞占比以及CD4^(+)/CD8^(+)比值低于Ⅰ期,CD8^(+)T细胞占比低于Ⅰ期,宫颈癌淋巴转移者CD4^(+)T细胞占比以及CD4^(+)/CD8^(+)比值低于无淋巴转移者,CD8^(+)T细胞占比水平高于无淋巴转移者,宫颈癌病灶直径≥4 cm者的外周血CD4^(+)T细胞占比及CD4^(+)/CD8^(+)比值均低于<4 cm者,低分化者癌灶组织内UBQLN1阳性检出率高于中高分化者,差异均有统计学意义(P<0.05)。随访至2022年1月,共29例宫颈癌患者确诊为复发,复发者外周血CD4^(+)T细胞占比以及CD4^(+)/CD8^(+)比值均低于未复发者,CD8^(+)T细胞占比以及UBQLN1阳性检出率均高于未复发者,差异有统计学意义(P<0.05)。绘制受试者操作特征(ROC)曲线提示,外周血免疫细胞群CD4^(+)/CD8^(+)比值在预测宫颈癌术后复发中的效能最高,其曲线下面积(area under curve,AUC)=0.951,95%CI(0.905~0.997),CD4^(+)、CD8^(+)、CD28^(+)T细胞单独应用时在预测宫颈癌术后复发时,CD4^(+)效能最高,其AUC=0.829,95%CI(0.746~0.912)。癌变
Objective To investigate the expression levels of immune cell group in peripheral blood and expression of ubiquilin 1(UBQLN1)in cervical cancer tissues,and also to explore the correlation between the expression of UBQLN1 and prognosis of cervical cancer patients.Methods Ninety⁃eight patients with cervical cancer who underwent radical resection combined with standardized postoperative treatment in Jincheng Grand Hospital from June 2018 to January 2020 were enrolled as the observation group,another 60 healthy women who received physical examination in the hospital were designated as the control group.Peripheral venous blood was collected before surgery to detect the expression levels of CD4^(+)T,CD8^(+)T and CD28^(+)T cells.Cervical cancer and adjacent normal tissues were collected,and immunohistochemistry was used to detect the expression level of UBQLN1.The patients had medical follow⁃ups till January 2022.Rcurrence rate of cervical cancer was statistically analyzed.Thereafter,the relationship between the expression of immune cell group in peripheral blood and expression levels of BUQLN1 in cervical cancer tissues with the clinicopathological characteristics and prognosis of the patients was analyzed.Results The proportion of CD8^(+)T cells in peripheral blood of the observation group was higher than that of the control group,the ratio of CD4^(+)/CD8^(+)was lower than that of the control group,and the positive rate of UBUQLN1 in cervical cancer tissues was higher than that in adjacent normal tissues,all with statistical significance(P<0.05).Peripheral blood CD4^(+)and CD4^(+)/CD8^(+)expression levels in stageⅡA cervical cancer were lower than those in stage I,and the proportion of CD8^(+)T cells was lower than that in stage I.The levels of CD4^(+)and CD4^(+)/CD8^(+)in cervical cancer patients with lymph node metastasis were lower than those without lymph node metastasis,while CD8^(+)level was higher than that without lymph node metastasis.The levels of CD4^(+)and CD4^(+)/CD8^(+)in peripheral blood of the pa
作者
马宁
张越
Ma Ning;Zhang Yue(Department of Gynaecology,Jincheng University Hospital,Shanxi Province,Jincheng 048000,China)
出处
《海军医学杂志》
2023年第3期279-284,共6页
Journal of Navy Medicine
关键词
宫颈癌
根治术
术后规范化治疗
复发
外周血免疫细胞群
泛素化蛋白1
Cervical cancer
Radical resection
Standardized postoperative treatment
Recurrence
Peripheral blood immune cell group
Ubiquilin 1
