摘要
本研究通过CRISPR/Cas9基因编辑技术对玉米ZmCYP72A5基因进行编辑,获得9个基因突变株系,分别命名为L1-L9,进而研究其在玉米生长发育中的功能。研究发现,突变植株平均高度明显降低,但植株节间数没有变化。进一步分析发现,突变植株的第9至11节间和节间细胞长度明显缩短,其抽穗时间延迟了2-5 d,叶片持绿时间延长,衰老变慢,但是籽粒鲜重和百粒重并没有降低。这些结果表明,该基因在玉米生物育种具有良好的应用潜力。
In this study,the gene editing(CRISPR/Cas9)technique was used to modify ZmCYP72A5 gene,so as to investigate its function in maize growth and development.Nine transgenic lines were obtained and named L1 to L9,respectively.The results showed that,compared with the control,the plant height of the transgenic plants was significantly decreased,and the total number of internodes per plant did not change.Further,the length of the 9th to 11th internodes and cells length in transgenic plants was significantly shortened.Moreover,the heading time was delayed by 2 to 5 days and leaf senescence was slowed down,but the fresh grain weight and 100 grain weight were not obviously reduced.Those results suggested that the modifying of ZmCYP72A5 gene could be useful for biological breeding of maize in the future.
作者
张文婷
王煜
赵丫杰
赵翔宇
ZHANG Wen-ting;WANG Yu;ZHAO Ya-jie;ZHAO Xiang-yu(State Key Laboratory of Crop Biology,College of Life Sciences/Shandong Agricultural University,Tai’an271018,China)
出处
《山东农业大学学报（自然科学版）》
北大核心
2023年第1期1-8,共8页
Journal of Shandong Agricultural University：Natural Science Edition
基金
山东省良种工程项目(2021LZGC022)
山东省泰山学者人才工程(ts201712024)。
关键词
玉米
调节基因
生物学性状
Maize
regulating gene
biological characteristics
