摘要
目的:建立丙戊酸(valproic acid, VPA)磁性分子印迹聚合物(VPA MMIPs)的固相分散萃取方法,并与气相色谱-质谱法(GC-MS)联用,评价VPA MMIPs的吸附性能。方法:(1)以VPA为模板分子,甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,乙腈为致孔剂,制备VPA MMIPs。通过傅里叶变换红外光谱(FTIR)、扫描电子显微镜(SEM)、透射电子显微镜(TEM)对材料进行表征。(2)通过对VPA MMIPs投加量、吸附时间及吸附测试液浓度的优化,确定最佳吸附条件并评价VPA MMIPs的特异性吸附能力。结果:(1)VPA MMIPs的制备条件为:模板分子/功能单体/交联剂的物质的量之比为1∶8∶40,致孔剂乙腈50 mL,引发剂偶氮二异丁腈(AIBN)50 mg,磁性材料0.40 g。SEM和TEM分析表明,制备的VPA MMIPs粒径分布为50~300 nm。FTIR显示VPA MMIPs材料合成成功。(2)吸附条件为:VPA MMIPs最佳投加量为20 mg,室温下吸附60 min。吸附特异性考察中,VPA MMIPs和丙戊酸磁性非分子印迹聚合物(VPA MNIPs)对VPA的最大平衡吸附量分别为3 423.68μg·g-1和1 403.81μg·g-1。结论:制备的VPA MMIPs对VPA具有较强的特异性吸附能力,该研究可为临床上VPA的检测提供新的思路。
OBJECTIVE To evaluate the ability of application of VPA MMIPs by establishing a dispersive solid phase extraction method based on VPA MMIPs coupled with gas chromatography coupled with mass spectrometry for the detection of VPA.METHODS VPA was selected as the template, and methacrylic acid, ethylene glycol dimethacrylate, azodiisobutyronitrile, acetonitrile and silanized modified Fe3O4were respectively used as the functional monomer, cross-linking agent, initiator, porogen and carrier to prepare VPA MMIPs.The reaction conditions were optimized to obtain the best parameters for the preparation of VPA MMIPs.The VPA MMIPs were characterized by fourier transform infrared spectroscopy, scanning electron microscopy, and transmission electron microscopy, respectively.The final conditions of absorption of VPA MMIPs were obtained by optimizing the dosages and absorption time of adsorbents and the concentration of adsorption test solution for evaluating the selective absorption capacity of VPA MMIPs.RESULTS The optimized preparation conditions were obtained as follows: the molar ratio of template molecule, functional monomer and crosslinking agent was 1∶8∶40;the porogen was 50 mL of acetonitrile;and the dosages of AIBN and magnetic material were respectively 50 mg and 0.40 g.The particle size distribution of VPA MMIPs was in the ranges of about 50-300 nm according to SEM and TEM analysis, and SEM image and FTIR showed that VPA MMIPs possessed an obvious core-shell structure and were successfully prepared.The optimized adsorption condition was obtained as follows: the optimal dosage of adsorbent was 20 mg, the adsorption time was 60 min at room temperature.In the investigation of adsorption selectivity, the maximum equilibrium adsorption capacity of VPA MMIPs and VPA MNIPs for VPA were respectively up to 3 423.68 μg·g-1and 1 403.81 μg·g-1.Compared with other commonly used clinical anti-epileptic drugs, VPA MMIPs have stronger selective adsorption capacity for VPA.CONCLUSION VPA MMIPs have stronger selective adsorpti
作者
范先鹏
张海燕
倪璐卿
高明
万树平
FAN Xian-peng;ZHANG Hai-yan;NI Lu-qing;GAO Ming;WAN Shu-ping(Department of Pharmacy,Wujin Hospital Affiliated with Jiangsu University,Jiangsu Changzhou 213000,China;The Wujin Clinical college of Xuzhou Medical University,Jiangsu Changzhou 213000,China;Department of Pharmacy,The Third People's Hospital of Changzhou,Jiangsu Changzhou 213000,China;Department of Pharmacy,Wujin District Traditional Chinese Medicine Hospital,Jiangsu Changzhou 213000,China)
出处
《中国医院药学杂志》
CAS
北大核心
2023年第4期356-362,共7页
Chinese Journal of Hospital Pharmacy
基金
常州市科技计划资助项目(编号:CJ20199001)。
