摘要
为探究脱落酸(Abscisic acid,ABA)对葡萄果实中单萜物质合成的影响,以玫瑰香型鲜食葡萄品种‘京香玉’为试材,在果实始熟前1周和始熟期用300 mg·L^(-1)ABA浸泡果实2次,测定处理后7、14、21、28和35 d果实中游离态单萜化合物含量以及ABA合成和信号转导通路、单萜合成通路中相关基因的表达量。在果实中共检测到28种单萜类化合物,并随果实的发育成熟不断积累。外源ABA处理的成熟果实中游离态单萜含量比对照提高45.20%。通过实时荧光定量PCR发现,ABA合成基因(VvNCED1、VvGT)在处理后7 d表达量明显高于对照,VvβG1相对表达量在处理后持续上升;ABA信号受体基因VvPYL1、信号转导相关基因VvPP2C2、VvSnRK2.1、VvABI5的转录水平分别在处理后21和35d明显高于对照;单萜合成通路上游关键酶基因(VvDXS2、VvDXS3、VvDXR、VvGPPS)以及单萜合酶基因VvPNLinNer的表达水平在处理后7d明显高于对照。根据以上结果,推测外源ABA处理可增强果实内部ABA信号的转导,进而引起单萜合酶底物水平的提升,加快向单萜类化合物的转变,改善玫瑰香型葡萄的品质。
In order to explore the effects of abscisic acid(ABA)on monoterpene synthesis in grape berries,this study used table grape species‘Jingxiangyu’with muscat flavor as experimental material,and soaked the clusters with 300 mg·L^(-1)ABA twice at one week before and during veraison.The contents of free monoterpenoids at 7,14,21,28 and 35 days after first treatment were analyzed qualitatively and quantitatively by GC–MS.The results showed that 28 kinds of monoterpenes were detected in berries,most of which accumulated during ripening.The total contents of free monoterpenoids in treated berries at maturity was 45.20%higher than that in the control,and the substance that changed most strongly was linalool.To investigate how ABA regulate monoterpenes synthesis,quantitative real-time PCR was used.The results indicated that the expression of ABA synthesis genes(VvNCED1 and VvGT)was much stronger than that of the control.The relative expression of VvβG1 increased after ABA treatment constantly.The transcriptional levels of ABA receptor VvPYL1 and signal transduction related genes VvPP2C2,VvSnRK2.1 and VvABI5 were distinctly higher than the control on 21 and 35 days after treatment respectively.The expression levels of upstream genes about monoterpene synthesis(VvDXS2,VvDXS3,Vv DXR,VvGPPS)and monoterpene synthase gene VvPNLinNer was significantly higher.According to the above results,it’s suggested that exogenous ABA enhance ABA signal transduction in berries,which lead to the increase of monoterpene substrate level and facilitate monoterpene synthesis,finally improve the quality of rose flavor grapes.
作者
王晓晨
聂子页
刘先菊
段伟
范培格
梁振昌
WANG Xiaochen;NIE Ziye;LIU Xianju;DUAN Wei;FAN Peige;LIANG Zhenchang(Beijing Key Laboratory of Grape Science and Enology,Key Laboratory of Plant Resources Institute,the LIA INNOGRAPE International Associated Laboratory,Institute of Botany,the Chinese Academy of Sciences,Beijing 100089,China;China National Botanical Garden,Beijing 100093,China;University of Chinese Academy of Sciences,Beijing 101400,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2023年第2期237-249,共13页
Acta Horticulturae Sinica
基金
国家重点研发计划项目(2018YFD1000205)。
关键词
葡萄
玫瑰香型
脱落酸
单萜
基因表达
grape
muscat flavor
abscisic acid
monoterpenoids
gene expression
