摘要
温室栽培对鲜食葡萄果实单萜积累的影响尚不明确。以早熟鲜食葡萄品种‘香妃’和‘早玫瑰香’为试材进行温室栽培,以露地栽培作为对照。采用顶空固相微萃取(HS–SPME)结合气相色谱—质谱联用(GC–MS)技术,辅助自动质谱图解卷积和鉴定系统(AMDIS),检测葡萄果实中游离态单萜类化合物的含量。共检测到29种来源于异戊二烯代谢途径的单萜类化合物。两个葡萄品种单萜类化合物总含量随果实发育均呈升高趋势,且露地栽培均高于温室栽培,果实成熟期‘香妃’高于‘早玫瑰香’。利用主成分分析(PCA)可以明显区分开温室和露地栽培下的‘早玫瑰香’,但两种栽培条件下‘香妃’的单萜化合物在成熟期愈加接近;整体趋势表现为露地栽培葡萄果实具有更丰富的柠檬烯、(Z)–别罗勒烯、(E,Z)–别罗勒烯、里那醇、β–月桂烯、异松油烯、(Z)–β–罗勒烯、cis和trans–氧化玫瑰、β–香茅醇和cis–吡喃型氧化里那醇。
The effect of greenhouse cultivation on accumulation of monoterpenes in table grapes remains unclear. The early ripening table grape varieties＇Xiangfei＇and＇Zao Meiguixiang＇were grown in the greenhouse and open-field as the control. Headspace solid phase micro-extraction（HS–SPME）and gas chromatography–mass spectrometry（GC–MS）combined with Automated Mass Spectral Deconvolution and Identification System（AMDIS）were employed to analyze the free monoterpenes in grape berries. A total of 29 monoterpenes from mevalonate pathway were identified. The total content of monoterpenes of the two varieties increased during berry development and＇Xiangfei＇grape had higher monoterpenes content than＇Zao Meiguixiang＇grape at maturity. The monoterpenes content of berries under open-field cultivation were significantly higher than that in greenhouse. The monoterpene of＇Zao Meiguixiang＇grape in greenhouse could be clearly distinguished from the control by principal component analysis（PCA）,but the ripening＇Xiangfei＇grape in greenhouse was close to the control. In general,grape berries under open-field cultivation produced higher content of limonene,（Z）-allo-ocimene,（E,Z）-allo-ocimene,linalool,β-myrcene,terpinolen,（Z）-β-ocimene,cis/trans rose oxide,β-citronellol and cis-pyran linalool oxide.
作者
孙磊
钱旭
张国军
闫爱玲
王晓玥
王慧玲
任建成
徐海英
SUN Lei;QIAN Xu;ZHANG Guojun;YAN Ailing;WANG Xiaoyue;WANG Huiling;REN Jiancheng;and XU Haiying＊(Beijing Academy of Forestry and Pomology Sciences,Beijing Engineering Research Center for Deciduous Fruit Trees,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops（North China）,Ministry of Agriculture,Beijing 100093,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2018年第8期1467-1478,共12页
Acta Horticulturae Sinica
基金
国家现代农业产业技术体系建设专项资金项目(CARS-29-yc-4)
北京市财政项目(KJCX20170111)
关键词
葡萄
温室
露地
单萜
积累
grape
greenhouse
open-field
monoterpene
accumulation
