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西印度醋栗枝叶提取物的α-葡萄糖苷酶抑制活性及安全性评价

α-Glucosidase Inhibitory Activity and Safety Evaluation of Branch and Leaf Extracts of Phyllanthus acidus
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摘要 对西印度醋栗(Phyllanthus acidus Skeels)枝叶提取物的α-葡萄糖苷酶抑制活性及安全性进行评价,为该植物的安全食用和药用研发提供参考。采用溶剂回流提取得到西印度醋栗枝叶的全组分样品,然后按照极性大小进一步对全组分进行分组划段,分别得到石油醚、乙酸乙酯和正丁醇组分,以及乙醇洗脱组分和水溶出组分;以半抑制浓度(IC_(50))为评价指标,比较不同组分的α-葡萄糖苷酶抑制活性。通过经典急毒实验法测定各组分对小鼠的近似最大耐受量(MTD)和半数致死量(LD_(50)),将全组分样品进行连续灌胃20 d评价其亚急性毒性。结果表明,西印度醋栗枝叶各组分对α-葡萄糖苷酶均有一定的抑制能力;全组分的MTD为2.0 g/kg,不同组分样品的毒性大小为乙醇洗脱组分>水溶出组分>石油醚组分>乙酸乙酯组分>正丁醇组分>全组分。主要急性毒性症状是惊跳随后怠动、扭体、呼吸急促等。亚急性毒性试验未见明显毒性反应及病变,表明在口服摄入西印度醋栗全组分2 g/kg/d(相当于干燥枝叶8.6 g/kg)是安全的,具有开发为降血糖、减肥食品和药品的潜力。 To provide reference for the safety consumption of Phyllanthus acidus(PA) as food and its further medicinal development and utilization,a comparative study on acute and short-termtoxicity and α-glucosidase inhibitory ability of different components of its branches and leaves was conducted.The crude extract of PA,prepared under aqueous ethanol reflux extraction,was partitioned successively with petroleum ether,ethyl acetate and N-butanol according to the polarity.Furthermore,an alcohol-eluted and water-eluted fractions were obtained from water-soluble residue by column chromatography.The half inhibitory concentration(IC_(50)) of α-glucosidase was used as the evaluation index to compare the in vitro hypoglycemic activity of different extracts.The acute toxicity of these fractions was screened by maximum tolerated dose(MTD),median lethal dose(LD_(50)) values,and their subacute toxicity was evaluated by continuous oral administration of samples for 20 days.The results showed that each component of PA had a certain inhibitory ability to α-glucosidase.The MTD of crude extract of PA was 2.0 g/kg,and the order of acute toxicity for different fractions was ethanol-eluted fraction>water-eluted fraction>petroleum ether fraction>ethyl acetate fraction>N-butanol fraction>crude extract.The main acute toxicity symptoms were startle followed by idling,writhing,and shortness of breath.Subacute toxicity test showed no obvious toxicity and lesions.It was indicated that it was safe to take 2 g/kg/d(equivalent to 8.6 g/kg of dried branches and leaves) of the whole component of PA at oral ingestion.Thus,they have the potential to be developed as hypoglycemic,weight-loss food and medicine.
作者 侯润庚 丁骁 杨力颖 张曼 张颖君 赵平 HOU Rungeng;DING Xiao;YANG Liying;ZHANG Man;ZHANG Yingjun;ZHAO Ping(Key Laboratory of State Forestry and Grassland Administration on Highly-efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming 650224,China;State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China;Yunnan Xinxing Greening Engineering Co.,Ltd.,Kunming 650228,China)
出处 《食品工业科技》 CAS 北大核心 2023年第7期252-259,共8页 Science and Technology of Food Industry
基金 云南省基础研究计划重点项目(202001AS070017) 国家自然科学基金项目(82074124)。
关键词 西印度醋栗 枝叶 Α-葡萄糖苷酶 急性毒性 亚急性毒性 安全性评价 Phyllanthus acidus branches and leaves α-glucosidase acute toxicity subacute toxicity safety evaluation
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