摘要
本文研究了多穗金粟兰(Chloranthus multistachys Pei)的化学成分及抗肿瘤活性。采用正相硅胶、反相、Sephadex LH-20凝胶和半制备高效液相等色谱技术进行分离纯化,根据波谱数据结合参考文献鉴定所得化合物的结构。从多穗金粟兰的乙酸乙酯部位中分离得到19个化合物,分别鉴定为:(12 R)-labda-8(17),13 E-dien-12,15,19-triol(1)、oplodiol(2)、长尾粗叶木内酯A(3)、ent-8(9)-pimarene-20-hydroxy-16-nor-15-oic acid(4)、3α,15β-dihydroxy-ent-kaur-16-ene(5)、inflexarabdonin J(6)、秦皮定(7)、6-羟基-7,8-二甲氧基香豆素(8)、N(N′-benzoyl-S*-phenylalaninyl)-S*-phenylalaninol benzoate(9)、N-benzoyl-L-phenylalaninol(10)、(R)-N-(1′-methoxycarbonyl-2′-phenylethyl)-4-hydroxybenzamide(11)、(E)-3-(benzo[d][1,3]dioxol-5-yl)-N-phenethylacrylamide(12)、flavokawain A(13)、stigmast-4-ene-3β,6β-diol(14)、β-谷甾醇(15)、cycloart-23-ene-3β,25diol(16)、水合蒎醇(17)、香草酸(18)、肉桂酸(19),除化合物3、12~18外,其余化合物均为首次从多穗金粟兰植物中分离得到。采用MTT法测试化合物1~19对人乳腺癌细胞株MCF-7、人前列腺癌细胞DU-145和人肺癌细胞A549的体外细胞毒活性,结果表明,化合物13、16和17对A549细胞的生长具有一定的抑制作用。
To study the chemical constituents of the medicinal plant Chloranthus multistachys Pei and their anti-tumor activity.The compounds were separated and purified by column chromatography such as silica gel,reverse phase column chromatography,Sephadex LH-20,preparative HPLC and other chromatographic separation methods.Their structures were identified by spectral analysis and comparison with the data in reported literatures.As aresult,nineteen compounds were isolated and their structures were identified as(12 R)-labda-8(17),13 E-dien-12,15,19-triol(1),oplodiol(2),lasianthuoactone A(3),ent-8(9)-pimarene-20-hydroxy-16-nor-15-oic acid(4),3α,15β-dihydroxy-ent-kaur-16-ene(5),inflexarabdonin J(6),fraxidin(7),6-hydroxy-7,8-dimethoxycoumarin(8),N(N′-benzoyl-S*-phenylalaninyl)-S*-phenylalaninol benzoate(9),N-benzoyl-L-phenylalaninol(10),(R)-N-(1′-methoxycarbonyl-2′-phenylethyl)-4-hydroxybenzamide(11),(E)-3-(benzo[d][1,3]dioxol-5-yl)-N-phenethylacrylamide(12),flavokawain A(13),stigmast-4-ene-3β,6β-diol(14),β-sitosterol(15),cycloart-23-ene-3β,25diol(16),sobrerol(17),vallinic acid(18),cinnamic acid(19)respectively,based on the spectral data,among the compounds 3,12-18,the remaining compounds were isolated from this plant for the first time.The cytotoxic activities of compounds 1-19 on human breast cancer cell line MCF-7,human prostate cancer cell line DU-145 and human lung cancer cell line A549 in vitro were tested by MTT assay.The results showed that compounds 13,16 and 17 had a certain inhibitory effect on the growth of A549 cells.
作者
朱成光
张卫青
张丽萍
梁伟
冯树慧
魏美琪
黄元射
晏晨
ZHU Cheng-guang;ZHANG Wei-qing;ZHANG Li-ping;LIANG Wei;FENG Shu-hui;WEI Mei-qi;HUANG Yuan-she;YAN Chen(Guizhou University of Traditional Chinese Medicine,Guiyang 550002,China;An Shun City People′s Hospital;An Shun University,Anshun 561000,China)
出处
《天然产物研究与开发》
CAS
CSCD
2023年第2期242-249,共8页
Natural Product Research and Development
基金
贵州省卫生健康委员会科学技术基金(gzwkj2021-440)
贵州省科技计划(黔科合支撑[2022]一般189)。
关键词
多穗金粟兰
化学成分
结构鉴定
抗肿瘤活性
Chloranthus multistachys Pei
chemical composition
structural identification
anti-tumor activity
