摘要
目的 以茶皂素为天然乳化剂制备黄芩苷纳米乳(baicalin nanoemulsion,Bai-NE),并探究其对C57BL/6小鼠毛发生长的促进作用。方法 采用超声乳化法制备Bai-NE,利用单因素实验法对Bai-NE的处方和制备工艺进行优化,并对最优处方和工艺制备的Bai-NE进行理化性质和稳定性考察。以C57BL/6小鼠为研究对象,评价Bai-NE对毛发生长的促进作用。结果 Bai-NE的最佳处方为茶皂素用量为0.5%,油相用量为5%,最佳制备工艺为超声功率180W,超声时间10min,超声次数1次。制得的Bai-NE为O/W型乳液,外观均一,平均粒径为(225.1±0.6)nm,多分散指数(polydispersity index,PDI)为0.082±0.031,载药量为1.10mg/g,pH值为7.25±0.20。稳定性结果显示,Bai-NE具有良好的离心稳定性和储存稳定性。Bai-NE能显著促进C57BL/6小鼠的毛发生长。结论 茶皂素可作为一种潜在的天然乳化剂用于Bai-NE的制备,制备的Bai-NE对C57BL/6小鼠的毛发生长有促进作用。
Objective To prepare baicalin nanoemulsion(Bai-NE) using tea saponins(TS) as a natural emulsifier, and to explore the promoting effect of Bai-NE on the hair growth of C57BL/6 mice. Methods The Bai-NE was prepared by phacoemulsification. The optimal prescription and process parameters were screened out by single factor test, and the physicochemical property and stability of the Bai-NE prepared by the optimal prescription and process parameters were studied. C57BL/6 mice were used to evaluate the effect of the Bai-NE on hair growth. Results The best prescription of the Bai-NE was 0.5% of tea saponin and 5% of oil phase. The best preparation technology was ultrasonic power 180 W, ultrasonic time 10 min and ultrasonic frequency one time. The prepared Bai-NE is an O/W lotion with uniform appearance, mean particle size of(225.1 ± 0.6) nm, polydispersity index(PDI) of 0.082 ± 0.031, drug loading of 1.10 mg/g, and pH of 7.25 ± 0.20. The stability results showed that the Bai-NE had good centrifugal stability and storage stability. Bai-NE can significantly promote the hair growth of C57BL/6 mice. Conclusion Tea saponin can be used as a potential natural emulsifier for the preparation of Bai-NE, which can promote the hair growth of C57BL/6 mice.
作者
陈莉萍
李小芳
何春容
洪雪梅
肖女
CHEN Li-ping;LI Xiao-fang;HE Chun-rong;HONG Xue-mei;XIAO Nyu(Xihua University,Chengdu 610039,China;Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;Western Theater General Hospital,Chengdu 610083,China)
出处
《中草药》
CAS
CSCD
北大核心
2023年第2期561-568,共8页
Chinese Traditional and Herbal Drugs
基金
四川省科技计划项目(2020YFS0463)
四川省中医药管理局科学技术研究专项项目(2021MS115)
四川中医药大健康产业发展与乡村振兴研究中心资助项目(DJKZC202202)。
关键词
茶皂素
黄芩苷
纳米乳
脂溢性脱发
药效学
超声乳化法
tea saponin
baicalin
nanoemulsion
seborrheic alopecia
pharmacodynamics
ultrasonic emulsification
