摘要
目的:对黄芩炭的炮制工艺及其指纹图谱进行研究,为黄芩炭质量控制模式的建立奠定基础。方法:正交试验及HPLC-UV指纹图谱。色谱条件:Hypersil C18柱(5.0 mm×200 mm,5μm),流动相甲醇-0.4%磷酸水溶液-乙腈线性梯度洗脱;流速1.0 mL.m in-1;检测波长277 nm。结果:不同产地正品黄芩通过规范炮制加工后得到的黄芩炭指纹图谱无明显差异。结论:通过正交试验确定的黄芩炭制工艺切实可行,指纹图谱测定方法重现性好,可为黄芩炭的规范化炮制及质量控制提供可靠的科学依据。
Objective： To investigate the process and HPLC fingerprint of Charred Radix Scutellariae, and lay a foundation of Charred Radix Scutellariae quanlity control mode. Method： Select HPLC-UV fingerprint. Chromatogram condition： Hypersil C18 column （5.0 mm× 200 mm, 5 μm）, mixtures of methanol, 0. 4% phosphoric acid and acetonitrile as mobile phase in a gradient mode. Flow rate： 1.0 mL · min; Detection wavelength was set at 277 nm. Result： There were no evident differences among Charred Radix Scutellariae that were normatively manufactured and processed. Condusion： This test proves the process is feasible, this method recurs well and can be used to provide scieitific basis for the normative process and quanlity control mode of Charred Radix Scutellariae.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第15期1518-1521,共4页
China Journal of Chinese Materia Medica
基金
河南省重大科技攻关项目(200422030700)
关键词
黄芩炭
正交试验
HPLC指纹图谱
Charred Radix Scutellariae
orthogonal test
HPLC fingerprint
