摘要
本试验旨在研究不同浓度孕马血清促性腺激素(Pregnant Mare Serum Gonadotropin,PMSG)和人绒毛膜促性腺激素(Human Chorionic Gonadotrophin,HCG)组合对家兔超数排卵的影响。以24只5月龄性成熟家兔为试验动物,将其随机分为4组,其中1组为对照组,另外3组分别注射50 IU、70 IU和90 IU的PMSG和定量为100 IU的HCG,注射后合笼交配并在24 h后对家兔进行解剖和冲胚观察,研究各组家兔的输卵管物理形态以及获得胚胎的质量与数量。结果显示,随着PMSG注射浓度的增大,输卵管形态变化不显著;充血卵泡数、畸形胚胎数呈增多趋势且组间差异极显著(P<0.01),获得的优质胚胎数呈现先增多后减少的趋势,且在注射70 IU+100 IU时获得最多213枚的优质胚胎。研究认为,不同浓度PMSG+HCG的超排组合不影响家兔输卵管物理形态,但超排效果有差异,随PMSG浓度的增大会使胚胎畸形率增高,建议在实际应用中PMSG+HCG的浓度以70 IU+100 IU为宜。
The purpose of this experiment is to study the effects of different concentrations of pregnant mare serum gonadotropin(PMSG)and human chorionic gonadotrophin(HCG)on superovulation in rabbits.Twentyfour five-month-old pubertal rabbits were randomly divided into four groups,one of which was the control group,the other three groups were injected with 50 IU,70 IU and 90 IU PMSG respectively and 100 IU HCG.After the injection,the rabbits were mated,and dissected and embryos removed 24 hours later.The physical state of fallopian tube and the quality and quantity of embryos were observed and analyzed.The results showed that with the increase of PMSG injection concentration,the change of fallopian tube was not significant;the number of congested follicles and abnormal embryos increased and the difference among the groups was significant(P<0.01),while the number of high-quality embryos increased first and then decreased.When PMSG+HCG injection was 70 IU+100 IU,213 high-quality embryos were obtained at most.The combination of superovulation at different concentrations of PMSG+HCG did not affect the physical fallopian tube morphology in rabbits,but the effect of superovulation was different.The rate of embryonic malformation increased with the increase of PMSG concentration.Therefore,it is recommended that the concentration of PMSG+HCG should be 70 IU+100 IU in practice.
作者
周娜
铁雅楠
杨雅楠
王欣荣
ZHOU Na;TIE Yanan;YANG Yanan;WANG Xinrong(College of Animal Science and Technology of Gansu Agricultural University,Lanzhou 730070)
出处
《中国农学通报》
2023年第2期93-97,共5页
Chinese Agricultural Science Bulletin
基金
甘肃农业大学特色学科开放基金资助项目(GAU-XKJS-2018-037)。
