摘要
目的 比较基于种植环境对次生代谢产物影响下的广陈皮与陈皮。方法 采用液质联用技术分析并指认不同品种之间陈皮样品中的差异成分,通过比较广陈皮与陈皮在283 nm下差异黄酮类成分之间的峰面积比值,分析不同产地陈皮中差异成分峰面积比值规律。结果 不同产地陈皮样品共有6个差异成分,分别为橙皮苷、甜橙黄酮、6-去甲氧基橘皮素、川陈皮素、橘皮素、5-去甲基川陈皮素。甜橙黄酮/6-去甲氧基橘皮素>0.60或橘皮素/5-去甲基川陈皮素>7.00为广陈皮,甜橙黄酮/6-去甲氧基橘皮素≤0.60或橘皮素/5-去甲基川陈皮素≤7.00为陈皮。结论 本研究采用283 nm波长下峰面积比值甜橙黄酮/6-去甲氧基橘皮素>0.60或橘皮素/5-去甲基川陈皮素>7.00作为广陈皮的判断依据,该方法可以提高陈皮产地区分指标的便捷性和普适性。
Objective To compare the effects of planting environment on secondary metabolites between Guangchenpi and Chenpi. Methods Liquid chromatography-mass spectrometry was used to analyze and identify the differential components in Chenpi samples from different varieties. The peak area ratio of the differential flavonoid components between Guangchenpi and Chenpi at 283 nm, the peak area ratio of the differential components in Chenpi from different regions was analyzed.Results There were six differential components Chenpi samples from different regions, including hesperidin, sinensetin,6-demethoxyhesperetin, nobiletin, hesperetin, and 5-demethylnobiletin. The ratio sinensetin/6-demethoxy-hesperidin>0.60 or hesperidin/5-demethoxyhesperidin>7.00 was Guangchenpi, and the ratio sinensetin/6-deme-thoxyhesperidin≤0.60 or hesperidin/5-demethoxyhesperidin ≤7.00 was Chenpi. Conclusion In this study, the peak area ratio of sinensetin/6-demethoxyhesperidin>0.60 or hesperidin/5-demethylnobiletin>7.00 at 283 nm wavelength is used as the basis for the determination of Guangchenpi, which can improve the convenience and universality of the classification index of Chenpi origin.
作者
石洪超
商雪莹
陈明权
罗卓雅
覃仁安
何风雷
孙东
SHI Hongchao;SHANG Xueying;CHEN Mingquan;LUO Zhuoya;QIN Ren’an;HE Fenglei;SUN Dong(Guangzhou Baiyunshan Pharmaceutical Co.Chenliji,Guangdong Province,Guangzhou510288,China;Guangdong Institute For Drug Control,Guangdong Province,Guangzhou510663,China)
出处
《中国医药导报》
CAS
2023年第3期104-108,117,共6页
China Medical Herald
基金
国家药品监督管理局药品快速检验技术重点实验室开放性课题(KF2021005)
广东省中药材产业化基地建设项目—陈李济新会陈皮产业化种植基地(粤工信消费函[2021]72号)。
关键词
陈皮
广陈皮
次生代谢产物
黄酮类成分
峰面积比值
Chenpi
Guangchenpi
Secondary metabolites
Flavonoids constituent
Peak area ratio
