摘要
目的 研究不同产地陈皮Citri Reticulatae Pericarpium化学成分的差异,结合化学模式识别方法对陈皮的产地进行判别分析。方法 采用Agilent SB-C_(18)(150 mm×3.0 mm,2.7μm)色谱柱分离,以乙腈-0.1%甲酸水溶液进行梯度洗脱,质谱采用电喷雾离子源,在正离子模式下采集数据。对色谱图中各色谱峰进行精确质量数识别,利用二级质谱裂解规律、PCDL数据库及对照品对化学成分进行定性分析。结合主成分分析、聚类分析、判别分析等多元统计方法分析了新会陈皮与其他不同产地陈皮的差异成分,并利用这些分析方法鉴别了未知产地陈皮的来源。结果 从不同产地陈皮中鉴定出了29个共有化学成分,主要为多甲氧基黄酮及黄酮苷类化合物。建立9个差异化学成分的判别模型,实现了新会陈皮的准确判别,准确率达94.4%。结论 可作为陈皮产地鉴别和质量控制的有效方法,为道地药材新会陈皮的判别提供参考。
Objective To analyze the differential chemical constituents in Chenpi(Citri Reticulatae Pericarpium) from different habitats. Then,habitats of Citri Reticulatae Pericarpium were discriminated by chemical pattern recognition methods. Methods The chromatographic separation was performed on an Agilent SB-C_(18) column(150 mm×3.0 mm,2.7 μm) with the mobile phase consisting of acetonitrile and 0.1% formic acid for gradient elution. The chromatographic effluent was detected and analyzed by UPLC-Q-TOF/MS in positive modes. Constituents were identified by PCDL database,fragmentation regularities,and comparing to standard samples. Then,Habitats difference analysis was carried out by principal component analysis,hierarchical cluster analysis and discriminant analysis. Results A total of 29 constituents were identified,mainly included polymethoxyflavones and flavonoid glycoside. Citri Reticulatae Pericarpium from XinHui was accurate discriminated by the created discriminant model with an accuracy of 94.4%. Conclusion This study provide an effectively method for the habitats distinctions of Citri Reticulatae Pericarpium,which is benefit to the identification of geo-authentic herbs for XinHui Citri Reticulatae Pericarpium.
作者
黄芳
周熙
罗辉泰
谢淑桐
张春华
谢梦婷
邓欣
吴惠勤
HUANG Fang;ZHOU Xi;LUO Hui-tai;XIE Shu-tong;ZHANG Chun-hua;XIE Meng-ting;DENG Xin;WU Hui-qin(Guangdong Engineering Research Center for Quality and Safety of Traditional Chinese Medicine,Guangdong Key Laboratory of Chemical Measurement and Emergency Test Technology,Institute of Analysis,Guangdong Academy of Sciences(China National Analytical Center,Guangzhou),Guangzhou 510070,China)
出处
《中草药》
CAS
CSCD
北大核心
2022年第20期6361-6368,共8页
Chinese Traditional and Herbal Drugs
基金
广州市科技计划项目(202102020530)。
