摘要
目的 分析乙肝肝硬化患者维生素D与肝功能及淋巴细胞亚群的相关性。方法 收集乙肝肝硬化患者89例,健康体检者92例(对照组),进行血清维生素D、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、白蛋白(ALB)、总胆红素(TBIL)、乙肝病毒脱氧核糖核酸(HBV DNA)含量及淋巴细胞亚群的检测,同时对乙肝肝硬化患者进行Child-Pugh评分。结果 乙肝肝硬化组患者血清维生素D含量低于对照组(P<0.001)。乙肝肝硬化患者血清维生素D含量与血清ALT、AST、TBIL、HBV DNA均无相关性,与ALB呈正相关(r=0.214,P=0.044),与Child-Pugh评分呈负相关(r=-0.300,P=0.004)。按照维生素D浓度分为3组后,乙肝肝硬化患者外周血CD3^(+)T细胞百分比、CD4^(+)T细胞百分比组间比较,差异均无统计学意义(P均>0.05)。CD8^(+)T细胞百分比组间比较差异有统计学意义(P=0.002),CD4^(+)/CD8^(+)比值组间比较,差异有统计学意义(P=0.002)。结论 乙肝肝硬化患者存在维生素D缺乏,且维生素D与乙肝肝硬化患者的肝功能异常、免疫功能紊乱相关,提示维生素D在乙肝肝硬化发展过程中可能具有免疫调节作用。
Objective To analyze the correlation between vitamin D and liver function,lymphocyte subsets in patients with chronic hepatitis B-associated liver cirrhosis. Methods A total of 89 patients with chronic hepatitis B-associated liver cirrhosis and 92 healthy subjects(control group)were enrolled. Serum vitamin D,alanine aminotransferase,aspartate aminotransferase,albumin,total bilirubin,hepatitis B virus nucleic acid and peripheral blood T lymphocyte subsets were detected. Meanwhile,Child-Pugh score was performed for patients with hepatitis B-associated liver cirrhosis. Results The vitamin D concentration in patients with chronic hepatitis B-associated liver cirrhosis was significantly lower than that in control group(P<0.001).There was no correlation between alanine aminotransferase,aspartate aminotransferase,total bilirubin,hepatitis B virus nucleic acid and vitamin D concentration in patients with chronic hepatitis B-associated liver cirrhosis.Vitamin D concentration in liver cirrhosis patients had a significant positive correlation with albumin(r=0.214,P=0.044),but a significant negative correlation with Child-Pugh score(r=-0. 300,P=0. 004). And further analysis of T lymphocyte subsets in chronic hepatitis B-associated liver cirrhosis patients which were divided into 3 groups according to vitamin D content suggested that the percentage of CD3^(+)T cells and CD4^(+)T cells were no significant differences(P all>0.05). The percentage of CD8^(+)T cells and the ratio of CD4^(+)/CD8^(+)changed significantly with vitamin D concentration(P=0.002). Conclusion Vitamin D deficiency is prevalent in the patients with chronic hepatitis B-associated liver cirrhosis. Serum vitamin D content is correlated with hepatocyte dysfunction and cellular immune disorder,suggesting that vitamin D may be involved in the regulation of immune function in patients with hepatitis B cirrhosis.
作者
马雯
李芹
郭媛
何瑞芬
朴文花
MA Wen;LI Qin;GUO Yuan;HE Ruifen;PIAO Wenhua(Center of Clinical Laboratory,Ningxia People’s Hospital/The First Affiliated Hospital of Northwest University for Nationalities,Yinchuan 750002,China;Department of Clinical Laboratory,The First Clinical Medical College of Northwest University for Nationalities,Yinchuan 750002,China)
出处
《宁夏医科大学学报》
2022年第12期1214-1218,共5页
Journal of Ningxia Medical University
基金
宁夏回族自治区人民医院培育振兴科研项目(201930)。
关键词
乙肝肝硬化
维生素D
肝功能
细胞免疫
hepatitis Bliver cirrhosis
vitamin D
liver function
cellular immune
