摘要
目的分析大鼠脑胶质瘤瘤周浸润区多模态MRI与相应病理学指标间的相关性,进而讨论胶质瘤瘤周浸润区MRI表现的病理及分子生物学基础。材料与方法以32只雌性Wistar大鼠为研究对象,通过微量进样器注射C6胶质瘤细胞建立大鼠原位脑胶质瘤模型,对照组注射等量不含肿瘤细胞的完全培养基。14 d后实验组及对照组行常规MRI及动脉自旋标记(arterial spin labeling,ASL)、弥散加权成像(diffusion weighted imaging,DWI)、磁共振波谱(magnetic resonance spectrum,MRS)检查;测定并计算胶质瘤中心区、瘤周浸润区相对表观弥散系数(relative apparent diffusion coefficient,rADC)、脑血流量(cerebral blood flow,CBF)、胆碱(choline,Cho)/氮-乙酰天冬氨酸(N-acetyl-aspartate,NAA)值。同时记录相应区域Ki-67值、血管内皮生长因子(vascular endothelial growth factor,VEGF)及CD105的微血管密度(microvessel density,MVD),将MRI结果(rADC值、CBF值)与相应病理学指标Ki-67、VEGF水平、CD105-MVD行独立样本t检验及Pearson相关分析。结果(1)肿瘤中心区、浸润区、对照组的rADC值、CBF值、Ki-67、VEGF水平、CD105-MVD组间比较存在差异,且差异具有统计学意义(P<0.05);(2)胶质瘤中心区Cho/NAA值大于对照组(P<0.001);(3)肿瘤中心区、瘤周浸润区rADC值与Ki-67呈负相关(r=-0.92、-0.74);(4)肿瘤中心区Cho/NAA值与Ki-67呈正相关(r=0.76),肿瘤中心区CBF与VEGF阳性率及CD105-MVD呈正相关(r=0.90,t=0.72);瘤周浸润区CBF与VEGF阳性率及CD105-MVD呈正相关(r=0.90、0.71)。结论本研究显示多模态MRI与相应病理学指标间具有一定相关性,利用多模态MRI可对脑胶质瘤以及瘤周浸润区的分子生物学行为进行初步评估,为临床进行肿瘤范围的评估、手术切除提供一定依据。
Objective:The characteristics of multimodal MRI in peritumoral infiltrating region of cerebral glioma and its correlation with corresponding pathological indicators were analyzed,so as to explore the molecularly biological basis of peritumoral infiltration in glioma.Materials and Methods:A total of 32 female Wistar rats were selected as research subjects,which were injected C6 glioma cells to established rat glioma models in situ by micro sampling syringe.The control group was injected with the same amount of complete culture medium without glioma cells.The rats were examined with conventional MRI sequences,arterial spin labeling(ASL),diffusion weighted imaging(DWI),magnetic resonance spectrum(MRS)after 14 days.The relative apparent diffusion coefficient(rADC),Cho/NAA were measured and calculated,and the cerebral blood flow(CBF)was measured.At the same time,the expression of Ki-67,vascular endothelial growth factor(VEGF)and microvessel density(MVD)of CD105 were recorded.The correlation between the rADC value,CBF and Ki-67,VEGF,CD105-MVD were analyzed.Results:(1)The differences in rADC,CBF,Ki-67,VEGF and CD105-MVD which between each two groups in the central region,the infiltrating region,the control group were statistically significant(P<0.05);(2)The Cho/NAA in the central area of glioma was higher than that in control group(P<0.05);(3)The rADC value were negatively correlated with Ki-67 in the central area of glioma and the infiltrating region(r=-0.92,-0.74);(4)The Cho/NAA was positively correlated with Ki-67 in the central area of glioma(r=0.76).There were positive correlations between the CBF and the expression of VEGF,CD105-MVD in the corresponding region(r=0.90,0.72).The CBF in the infiltrating region had positive correlations with the expression of VEGF and CD105-MVD(r=0.90,0.71).Conclusions:This study revealed multimodal MRI was correlated with relative pathological indicators,and multimodal MRI was helpful to preliminary evaluate the molecularly biological characteristics in glioma and peritumoral infiltr
作者
涂颖珊
翁爱婷
任安利
董鹏
TU Yingshan;WENG Aiting;REN Anli;DONG Peng(Department of Radiology,Fuzhou Second Hospital,Fuzhou,350007,China;School of Medical Imaging,Weifang Medical University,Weifang 261053,China)
出处
《磁共振成像》
CAS
CSCD
北大核心
2023年第1期105-110,共6页
Chinese Journal of Magnetic Resonance Imaging
基金
山东省自然科学基金(编号:ZR2014HL083)。
关键词
脑胶质瘤
瘤周浸润
磁共振成像
多模态磁共振成像
动脉自旋标记
弥散加权成像
磁共振波谱
大鼠
病理学
cerebral glioma
peritumoral infiltration
magnetic resonance imaging
multimodal magnetic resonance imaging
arterial spin labeling
diffusion weighted imaging
magnetic resonance spectrum
rat
pathology
