摘要
目的探讨ATP结合盒转运蛋白G1(ABCG1)介导的胆固醇外流在系统性红斑狼疮(SLE)患者中的变化及临床意义。方法选取2017年1月至2021年10月在青岛市市立医院(下称该院)就诊的SLE患者73例作为SLE组,其中合并心血管并发症患者40例,未合并心血管并发症患者33例,另选取同期在该院进行健康体检的52例体检健康者作为健康对照组,通过构建ABCG1高表达质粒载体转染中国仓鼠卵巢细胞(CHO-K1),荧光标记法检测SLE组和健康对照组胆固醇外流情况,计算胆固醇流出率,统计学分析ABCG1介导胆固醇外流的变化。结果与CHO-K1细胞相比较,在ABCG1表达水平上调的CHO-K1细胞中,健康对照组、SLE组未合并心血管并发症及合并心血管并发症的胆固醇外流率均显著增加,差异有统计学意义(P<0.05);在CHO-K1细胞中,与健康对照组相比较,SLE组胆固醇外流率明显增高,差异有统计学意义(P<0.001),与未合并心血管并发症的SLE组相比较,SLE合并心血管并发症组胆固醇外流率明显增高,差异有统计学意义(P<0.05);在ABCG1高表达的CHO-K1细胞中,与健康对照组相比较,SLE组胆固醇外流率明显增高,差异有统计学意义(P<0.05),与未合并心血管并发症的SLE组相比较,SLE合并心血管并发症组胆固醇外流率明显增高,差异有统计学意义(P<0.05)。结论ABCG1介导的胆固醇外流途径在SLE患者及合并心血管并发症的SLE患者中均发生明显变化,可能与SLE患者中心血管疾病高发具有一定的关系。
Objective To investigate the changes of ATP binding cassette transporter G1(ABCG1)-mediated cholesterol efflux in patients with systemic lupus erythematosus(SLE)and its clinical significance.Methods A total of 73 patients with SLE diagnosed in Qingdao Municipal Hospital(hereinafter referred to as the hospital)from January 2017 to October 2021 were selected as the SLE group,including 40 patients with combined cardiovascular complications,33 patients without combined cardiovascular complications.Another 52 healthy people undergoing physical examination in the hospital at the same time were selected as the healthy control group.Chinese hamster ovary cells(CHO-K1)were transfected by constructing ABCG1 high expression plasmid vector,cholesterol efflux was detected by fluorescent labeling method in SLE group and health control group,and cholesterol efflux rate was calculated.The changes of ABCG1-mediated cholesterol efflux were statistically analyzed.Results Compared with CHO-K1 cells,in CHO-K1 cells with up-regulated ABCG1 expression,the cholesterol efflux rate was significantly increased in the healthy control group,SLE group without and with cardiovascular complications,and the differences were statistically significant(P<0.05).In CHO-K1 cells,the cholesterol efflux rate was significantly increased in the SLE group compared with the healthy control group(P<0.001).Compared with SLE group without cardiovascular complications,the cholesterol outflow rate in SLE group with cardiovascular complications was significantly higher,and the difference was statistically significant(P<0.05).In CHO-K1 cells with high ABCG1 expression,the cholesterol efflux rate was significantly increased in the SLE group compared with the healthy control group,and the difference was statistically significant(P<0.05).Compared with the SLE group without cardiovascular complications,the cholesterol outflow rate in the SLE group with cardiovascular complications was significantly higher,and the difference was statistically significant(P<0.05).Conclu
作者
寇艳婷
朱星星
李莉
原江水
KOU Yanting;ZHU Xingxing;LI Li;YUAN Jiangshui(Graduate School,Dalian Medical University,Dalian,Liaoning 116044,China;Department of Clinical Laboratory,Qingdao Municipal Hospital,Qingdao,Shandong 266071,China;Department of Clinical Medical,Qingdao Medical College,Qingdao University,Qingdao,Shandong 266073,China)
出处
《国际检验医学杂志》
CAS
2023年第1期24-28,共5页
International Journal of Laboratory Medicine
基金
国家自然科学基金青年项目(31800680)。
关键词
系统性红斑狼疮
胆固醇外流
ATP结合盒转运蛋白G1
高密度脂蛋白胆固醇
systemic lupus erythematosus
cholesterol efflux
ATP-binding cassette transporter protein G1
high density lipoprotein cholesterol
