摘要
目的探讨大鼠高位脊髓损伤急性期心肌组织自主神经活性物质的变化。方法清洁级健康雄性SD大鼠24只,8~10周龄,体重250~300 g,采用随机数字表法分为假手术组(6只)和脊髓损伤组(18只),脊髓损伤组又分为伤后4,12,24 h 3个时间点,每个时间点6只。用改良Allens打击法建立大鼠高位脊髓损伤模型,假手术组仅暴露脊髓。观察和记录各组术后表现,采用BBB评分法评估大鼠下肢运动功能。各组分别于伤后各时间点取心肌组织,透射电镜下观察心肌细胞超微结构;采用Western blot法和RT-PCR检测心肌酪氨酸羟化酶(TH)、去甲肾上腺素转运蛋白(NET)、乙酰胆碱酯酶(AChE)和乙酰胆碱转移酶(ChAT)蛋白及mRNA的表达水平。结果假手术组术后四肢活动正常,与术前相比未见明显变化;BBB评分均为21分。脊髓损伤组活动和进食显著减少,双下肢呈弛缓性瘫痪,无自主排泄;BBB评分伤后4,12 h均为0分,伤后24 h评分稍有升高,最高为1分。假手术组心肌细胞超微结构未见明显异常,脊髓损伤组心肌细胞超微结构出现不同程度的改变。Western blot显示,与假手术组比较,脊髓损伤组伤后不同时间点心肌组织TH和NET蛋白表达下降,AChE和ChAT蛋白表达升高(P<0.05或0.01)。RT-PCR显示,与假手术组比较,脊髓损伤组伤后不同时间点心肌组织TH和NET mRNA表达下降,AChE和ChAT mRNA表达升高(P<0.05或0.01)。脊髓损伤组伤后24 h与4,12 h比较,TH、NET mRNA差异有统计学意义(P均<0.05);伤后12,24 h与4 h比较、伤后24 h与12 h比较,ChAT mRNA差异有统计学意义(P均<0.05)。结论大鼠高位脊髓损伤急性期心肌组织交感神经活性物质TH和NET减少,迷走神经活性物质AChE和ChAT增多,这可能与高位脊髓损伤后阻断高级中枢对心脏的交感神经支配,导致副交感神经相对兴奋有关。
Objective:To investigate the changes of autonomic nervous active substances in myocardium of rats with acute high-level spinal cord injury.Methods:Twenty-four clean-level healthy adult male SD rats weighting 250-300 g for 8-10 weeks old were divided into control group(n=6)and spinal cord injury group(n=18)according to the random number table.The spinal cord injury group was subdivided at 4,12 and 24 hours,with 6 rats at each time point.The high-level spinal cord injury model was established by the modified Allen′s weight-drop method,and the spinal cord was only exposed in control group.The postoperative performance and BBB score for limb movement were observed in each group.The myocardium of each group was resected and used to observe ultrastructure of myocardial cells under transmission electron microscope and detect protein and mRNA levels of tyrosine hydroxylase(TH),noradrenaline transporter(NET),acetylcholinesterase(AChE)and choline acetyltransferase(ChAT)by Western blot and RT-PCR analysis.Results:Rats of control group showed normal limb motion after operation without significant change from the preoperation level,and mean BBB score was 21 points.Rats of spinal cord injury group showed significantly reduced activities and feeding,with flaccid paralysis of both lower limbs as well as no spontaneous excretion,and showed BBB score of 0 point at 4 hours and 12 hours after injury,which was increased slightly at 24 hours after injury,with the highest score for 1 point.The ultrastructure of myocardial cells showed no obvious abnormalities in control group,while different degrees of changes in spinal cord injury group.Compared with control group,Western blot analysis showed that protein levels of TH and NET were decreased,while AChE and ChAT were increased in spinal cord injury group(P<0.05 or 0.01).Compared with control group,RT-PCR analysis showed that mRNA levels of TH and NET were decreased,while AChE and ChAT were increased in spinal cord injury group(P<0.05 or 0.01).mRNA levels of TH and NET in spinal cord i
作者
魏丽钦
廖烨
李佳琦
张冰玉
黄钦凤
张良成
陈辉
Wei Liqin;Liao Ye;Li Jiaqi;Zhang Bingyu;Huang Qinfeng;Zhang Liangcheng;Chen Hui(Department of Anesthesiology,Fujian Medical University Union Hospital,Fuzhou 350001,China;Department of Anesthesiology,First Afiliated Hospital of Fujian Medical University,Department of Anesthesiology,National Regional Medical Center,Binhai Campus of First Affiliated Hospital,Anesthesiology Research Institute of Fujian Medical University,Fuzhou 350005,China)
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2022年第11期1036-1041,共6页
Chinese Journal of Trauma
基金
福建医科大学启航基金(2020QH1056)。
关键词
心肌
脊髓损伤
自主神经系统
Myocardium
Spinal cord injuries
Autonomic nervous system
