摘要
目的:研究大鼠高位脊髓损伤并创伤失血休克后急性期血流动力学及血清去甲肾上腺素(nore-pinephrine,NE)、肾上腺素(epinephrine,E)、神经肽Y(neuropeptide Y,NPY)的变化规律。方法:将50只SD大鼠随机平均分为假手术组(A组)、失血组(B组)、脊髓损伤组(C组)、脊髓损伤并失血组(D组)和多发伤组(脊髓损伤、失血并单侧胫骨骨折,E组)。失血量为大鼠全身血量的20%,动脉瘤夹法制成C7～T1节段完全性脊髓损伤。监测伤后6h内不同时间点血流动力学变化。另取40只SD大鼠复制上述B、C、D、E组模型(每组均为10只),于损伤前及伤后不同时间点测定血清NE、E浓度(酶联免疫吸附法)和血浆NPY浓度(放射免疫测定法)。结果:E组大鼠平均动脉压在伤后1～6h,心率在伤后即刻、0.5、4、5、6h,左室收缩压在伤后3～6h,左心室内压最大变化速率在伤后0.5～6h显著低于其他各组(P<0.05)。E组大鼠血清E浓度在伤后0.5、1、2、3、5h,NE浓度在伤后2、3、6h轻度高于伤前及C组(P<0.05);NPY浓度在伤后0.5～2h和5～6h呈小幅双峰增长(和伤前及C组比,P<0.05)。E组伤后各时间点血NE、E、NPY浓度与D组比较差异无显著性(P>0.05),均远低于B组(P<0.05)。结论:大鼠高位脊髓损伤并创伤失血休克后急性期血流动力学出现严重紊乱,血NE、E、NPY浓度相对缺乏。
Objective：To investigate the change of hemodynamics and the concentration of serum nore-pinephrine（NE） ,epinephrine（E）,neuropeptide Y（NPY） in trauma-hemorrhage shock rats combined with spinal cord injury in vivo during acute stage.Method：Fifty Sprague-Dawley rats were randomized averagely to 5 experimental groups：sham-operation（group A）,animals with hemorrhage（group B）,animals with spinal cord injury （group C）,animals combined with spinal cord injury and hemorrhage （group D）,and animals combined with spinal cord injury, hemorrhage and tibia fracture（group E）.The amount of hemorrhage was 20% rat＇s total body volume.Aneurysm clip was employed to make complete spinal cord injury at C7-T1.Hemodynamics were monitored at before trauma and 0~6h after trauma.Forty Sprague-Dawley rats were randomized averagely to 4 experimental groups to copy the way of vulnerate in group B,C,D,E to measure the concentration of serum NE,E （ELISA）,plasma NPY （radioimmunity） at different time points.Result：Mean arterial pressure （MAP） of group E in 1-6h,heart rate（HR） in 0,0.5,4,5,6h,left ventricular systolic pressure（LVSP） in 3- 6h,left ventricular pressure（±dp/dtmax） in 0.5-6h drop dramatically compared with other groups （P〈0.05 ）.The concentration of E in 0.5,1,2,3,5h,NE in 2,3,6h rise slightly compared with normal and group C（P〈0.05）. The concentration of NPY exhibited two peaks：the first rise emerges between 0.5-2h and the second between 5-6h after trauma（compared with normal and group C,P〈0.05）.The concenntrations of NE,E,NPY of group E had no statistics difference compared with group D （P〉0.05）,but lower than group B dramatically （P〈0.05）. Conclusion：The trauma-hemorrhage shock rats combined with spinal cord injury exhibited dramatic disorder in hemodynamics and deficiency of NE,E,NPY in blood during acute stage.
出处
《中国脊柱脊髓杂志》
CAS
CSCD
2008年第1期64-69,共6页
Chinese Journal of Spine and Spinal Cord
关键词
脊髓损伤
大鼠
血流动力学
多发伤
Spinal cord injury
Rats
Hemodynamics
Muhiple trauma
