摘要
目的探讨磷酸肌酸钠对高位脊髓损伤后心肌损伤保护作用以及对心肌钙敏感受体（calcium-sensing receptor，CaSR）表达的影响。方法健康雄性SD大鼠30只，体重250～300g。按随机数字表法分为：假手术组、脊髓损伤12h组（损伤I组）、脊髓损伤24h组（损伤Ⅱ组）、脊髓损伤12h腹腔一次性注射磷酸肌酸钠（治疗I组）、脊髓损伤24h腹腔一次性注射磷酸肌酸钠（治疗Ⅱ组），每组6只。采用10g砝码从5cm高处沿中空玻璃管垂直自由落下撞击C，脊髓制备高位脊髓损伤模型。测定血清肌钙蛋白I（cTnI）含量；取大鼠心肌组织，透射电镜下观察心肌细胞超微结构；荧光定量PCR检测CaSR mRNA；Western blot检测心肌组织CaSR蛋白。结果损伤I、Ⅱ组cTnI含量分别为（0.031±0.002）U／L、（0.0264±0.001）U／L，治疗I、Ⅱ组分别为（0.0234±0.002）U／L、（0.018±0.006）U／L，均高于假手术组（0.0044±0.002）U／L（P〈0.05）；治疗组均低于相同时相点损伤组（P〈0.05）。损伤I、Ⅱ组CaSRmRNA表达分别为（0.991±0.146）×10-3、（1.245±O.204）×10-3，治疗I、Ⅱ组分别为（0.880±0.096）×10-3、（0.782±O.138）×10-3，均高于假手术组（0.4374±0.065）×10-3（P〈0.05）；治疗组均低于相同时相点损伤组（P〈0.05）。损伤I、Ⅱ组CaSR蛋白表达分别为（0.627±0.066）×10-3、（0.809±O.154）×10-3，治疗I、Ⅱ组分别为（0.505±0.176）×10-3、（0.5244±0.138）×10-3，均高于假手术组（0.3314±0.102）×10-3（P〈0.05）；治疗组均低于相同时相点损伤组（P〈0.05）。电镜结果显示，假手术组心肌超微结构正常，损伤组和治疗组各时相点均出现心肌损伤的超微结构改变，但治疗组心肌细胞损伤明显比相同时相点损伤组轻。结论磷酸肌酸钠可明显抑制高位脊髓损伤血清eTnI含量升高，减轻心肌组织超微结构的损伤性改变以及减少心肌细胞CaSR的表达
Objective To investigate the effect of ereatine phosphate sodium on myocardial protection and calcium-sensitive receptor （CaSR） expression following high-level spinal cord injury. Methods Thirty healthy male SD rats weighing 250-300 g were assigned to sham operation, 12-hour injury, 24-hour injury, 12-hour injury followed by a single intraperitoneal injection of ereatine phosphate sodium, and 24-hour injury followed by a single intraperitoneal injection of creatine phosphate sodium according to the random number table, with 6 rats in each group. High-level spinal cord injury was induced at C7 segment by dropping a 10 g weight falling freely along the hollow glass tube from a 5 cm height. Level of blood troponin I （cTnI） was measured. Myocardial tissues were collected to study ultrastrueture of myocardial cells under transmission electron microscope and CaSR expression using fluorescence quantitative PCR andWestern blotting. Results cTnI level was （0.031±0.002 ） U/L and （0.026±0.001） U/L in 12- and 24-hour injury groups, but it was reduced to （0.023±0.002） U/L and （0.018±0.006） U/L at the same time point in treatment groups （ P 〈 0.05 ）. Whereas either in injury or treatment groups, cTnI level was higher than （ 0.004±0.002 ） U/L in sham operation group （ P 〈 0.05 ）. CaSR mRNA level was （0.991±0.146）×10 -3 and （ 1.245±0.204）× 10 -3 in 12- and 24-hour injury group and decreased to （0.880±0.096）×10-3 and （0.782±0.138）× 10 -3 at the same time point in treatment groups （P 〈 0.05 ）, but all were higher than （0.437±0.065 ）×10 -3 in sham operation group （ P 〈 0.05 ）. CaSR protein expressed in 12- and 24-hour injury group was （ 0.627±0.066）×10-3 and （0.809±0.154）×10-3 and lowered to （0.505±0.176）×10-3 and （0.524±0.138）×10-3 at the same time point in treatment groups, but all were higher than （0.331±0.102 ）×10-3 in sham operation group （P 〈 0.05 ）. Transmission electron microscopy demonstrated normal my
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2014年第10期1046-1049,共4页
Chinese Journal of Trauma
基金
福建省卫生厅创新课题资助项目（2009-CXB-24）
关键词
心肌
脊髓损伤
受体
钙敏感
磷酸肌酸钠
Myocardium
Spinal cord injuries
Receptors
calcium-sensing
Phosphocre-atine
