摘要
目的研究水通道蛋白1(AQP1)、微小RNA-3195(miR-3195)、PINCH-1在喉癌中的表达及作用机制。方法选取2019年5月至2020年11月收治的81例喉癌患者,对患者癌组织、癌旁组织标本进行免疫组化染色,荧光定量PCR检测AQP1、miR-3195、PINCH-1表达,对比不同病理特征喉癌组织中AQP1、miR-3195、PINCH-1表达情况,分析AQP1、miR-3195、PINCH-1表达在喉癌组织中的相关性。结果喉癌患者癌组织中AQP1、PINCH-1表达高于癌旁组织,miR-3195表达低于癌旁组织(P<0.05)。中低分化程度患者AQP1、PINCH-1表达高于高分化程度患者,miR-3195表达低于高分化程度患者(P<0.05);肿瘤浸润深度为T1~T2期患者AQP1、PINCH-1表达低于T3~T4期患者,miR-3195表达高于T3~T4期患者(P<0.05);TMN分期为Ⅰ~Ⅱ期患者AQP1、PINCH-1表达低于Ⅲ~Ⅳ患者,miR-3195表达高于Ⅲ~Ⅳ患者(P<0.05),淋巴结转移患者AQP1、PINCH-1表达高于淋巴结未转移患者,miR-3195表达低于淋巴结未转移患者(P<0.05)。AQP1、miR-3195、PINCH-1联合检测对喉癌的预测价值优于单一检测。喉癌组织中AQP1、miR-3195呈负相关(r=-0.476,P=0.001);AQP1、PINCH-1呈负相关(r=-0.645,P=0.001);miR-3195、PINCH-1呈正相关(r=0.523,P=0.001)。结论AQP1、miR-3195、PINCH-1在喉癌中的表达出现异常,且AQP1、miR-3195、PINCH-1表达变化与喉癌患者分化程度、肿瘤浸润深度、TNM分期、淋巴结转移情况相关,三者表达之间具有一定相关性,共同参与喉癌的发展过程。
Objective To investigate the expression and action mechanism of aquaporin 1(AQP1),microrNA-3195(Mir-3195)and PINCH-1 in laryngeal carcinoma.Methods A total of 81 patients with laryngeal cancer who were treated in our hospital were enrolled in the study.Immunohistochemical staining was performed for the tumor tissues and para-cancerous tissues of the patients,and the expression levels of AQP1,Mir-3195 and PINCH-1 were detected by fluorescence quantitative PCR.The expressions of AQP1,Mir-3195 and PINCH-1 in laryngeal cancer tissues with different pathological features were observed and compared,and the correlation between the expressions of AQP1,Mir-3195 and pinCH-1 in laryngeal cancer tissues was analyzed.Results The expression levels of AQP1 and PINCH-1 in cancer tissues of patients with laryngeal cancer were significantly higher than those in para-cancerous tissues,however,the expression levels of Mir-3195 were significantly lower than those in para-cancerous tissues(P<0.05).The expression levels of AQP1 and PINCH-1 in patients with medium and low differentiation were significantly higher than those of patients with high differentiation,but,the expression levels of Mir-3195 were significantly lower than those in patients with high differentiation(P<0.05).The expression levels of AQP1 and PINCH-1 in patients with T1-T2 stages were significantly lower than those in patients with T3-T4 stages,however,the mir-3195 levels were significantly higher than those in patients with T3-T4 stages(P<0.05).The expression levels of AQP1 and PINCH-1 in patients with TMN stageⅠ~Ⅱwere significantly lower than those in patients with stageⅢ~Ⅳ,but the expression levels of Mir-3195 were were significantly higher than those in patients with stageⅢ~Ⅳ(P<0.05).Moreover the expression levels of AQP1 and PinCH-1 in patients with lymph node metastasis were were significantly higher than those in patients without lymph node metastasis,however,the expression levels of Mir-3195 were significantly lower than those in patients without
作者
陶维平
李琴
李湘胜
TAO Weiping;LI Qin;LI Xiangsheng(Department of Otorhinolaryngology,The Fourth Hospital of Changsha City,Hunan,Changsha 410006,China)
出处
《河北医药》
CAS
2022年第22期3389-3392,共4页
Hebei Medical Journal
基金
湖南省卫生健康委科研计划项目(编号:20200004)。
关键词
喉癌
癌组织
水通道蛋白1
淋巴结转移
分化程度
laryngeal cancer
cancerous tissue
aquaporin 1
lymph node metastasis
differentiation degree
