摘要
目的研究前B细胞克隆增强因子(PBEF)对缺氧/复氧(H/R)处理的非小细胞肺癌(NSCLC)细胞生长及水通道蛋白1(AQP1)和钠通道蛋白α(ENaCα)表达的影响。方法将NSCLC A549细胞分为4组:对照组(C组)、H/R处理模型组(M组)、H/R处理+pCAGGS空载组(pCAGGS NC组)、H/R处理+pCAGGS PBEF组(pCAGGS PBEF组);通过构建pCAGGS PBEF质粒并转染到细胞中建立H/R A549细胞培养实验模型。采用qPCR和Western blot(WB)检测PBEF的表达;采用WB检测AQP1和ENaCα的表达;采用流式细胞术检测细胞凋亡。结果M组细胞凋亡率显著高于C组(P<0.05),pCAGGS PBEF组细胞凋亡率显著高于pCAGGS NC组(P<0.05),M组和pCAGGS NC组细胞凋亡率比较差异无统计学意义(P>0.05)。pCAGGS PBEF组细胞中AQP1和ENaCα的蛋白表达水平显着低于pCAGGS NC组(P<0.05),而C组、M组、pCAGGS NC组3组细胞中AQP1和ENaCα的蛋白表达水平比较差异均无统计学意义(P>0.05)。结论PBEF过表达促进了H/R处理的NSCLC A549细胞凋亡,并抑制AQP1和ENaCα蛋白的表达,提示PBEF在低氧环境中可能具有辅助治疗作用。
Objective To investigate the effects of pre-B cell colony-enhancing factor(PBEF)on growth of hypoxia/reoxygenation(H/R)-treated human non-small cell lung carcinoma(NSCLC)cells and expression of aquaporin-1(AQP1)and epithelial sodium channelα-subunit(ENaCα).Methods NSCLC A549 cells were divided into four groups:control group(C group),H/R-treated model group(M group),H/R treatment+empty pCAGGS vector group(pCAGGS NC group),and H/R treatment+pCAGGS PBEF group(pCAGGS PBEF group).PCAGGS PBEF plasmids were constructed and transfected into cells to establish the H/R model,and levels of PBEF expression were detected by qPCR and Western blot(WB).The expression AQP1 and ENaCαwas determined by WB,and cell apoptosis was detected by flow cytometry.Results The apoptosis rate in M group was higher than that in C group,and that in pCAGGS PBEF group was higher than that in pCAGGS NC group(P<0.05).The difference in apoptosis rate was not significant between M group and pCAGGS NC group(P>0.05).The expression of AQP1 and ENaCαin pCAGGS PBEF group was lower than that in pCAGGS NC group(P<0.05).There were no significant differences in AQP1 and ENaCαexpression among C,M and pCAGGS NC groups(P>0.05).Conclusion PBEF overexpression can promote apoptosis and inhibit AQP1 and ENaCαexpression in H/R-treated NSCLC A549 cells,suggesting that PBEF may play an adjuvant therapeutic role in hypoxic environment.
作者
尤苗苗
张祖磊
许伟长
芦潮
龚艺
杨威
YOU Miao-miao;ZHANG Zu-lei;XU Wei-chang;LU Chao;GONG Yi;YANG Wei(Department of Thoracic Surgery,Jiangxi Chest Hospital,Nanchang 330006,China;Medical Department of Graduate School,Nanchang University,Nanchang 330006,China;Department of Cardiovascular Surgery of the Second Affiliated Hospital,Nanchang University,Nanchang 330006,China;Department of Cardiovascular Surgery,the First Affiliated Hospital of Gannan Medical College,Ganzhou 341000,China)
出处
《南昌大学学报（医学版）》
CAS
2020年第4期10-14,22,共6页
Journal of Nanchang University：Medical Sciences
基金
江西省青年科学基金项目(20161BAB215236)。
关键词
前B细胞克隆增强因子
缺氧/复氧
非小细胞肺癌
水通道蛋白1
钠通道蛋白α
pre-B cell colony-enhancing factor
hypoxia/reoxygenation
non-small cell lung carcinoma
aquaporin-1
epithelial sodium channelα-subunit
