摘要
【背景】‘爱媛28’橘橙是杂交柑橘品种(Citrus nanko×C.amakusa),因其优良的果实品质,受到人们的青睐。疏果是农业生产过程中常用的一种提高果实品质的技术,不仅能使果实体积明显增大,而且还能够增加果实可溶性固形物含量。但是,疏果增加果实可溶性固形物含量的具体机制仍然不清楚。【目的】本研究以枳(Poncirus trifoliata)为基砧、椪柑(Citrus reticulata)为中间砧的4年生‘爱媛28’橘橙作为试验材料,探索疏果影响果实糖酸含量变化的具体机制。【方法】在‘爱媛28’果实幼果期进行疏果,每隔半个月左右测定疏果果实与未疏果果实的横纵茎,采样并测量单果重与糖酸含量,选择糖酸含量差异明显的时期测定糖酸代谢相关酶的活性及其对应编码基因的相对表达量。【结果】疏果显著增加‘爱媛28’果实在生长中后期的横纵茎和单果重,显著加快果实柠檬酸含量的降解速度,但并未影响果实成熟时最终的柠檬酸含量;显著增加了果实的葡萄糖和蔗糖含量,但对果糖含量没有显著影响。疏果显著增强蔗糖代谢过程中的蔗糖合成酶SSⅡ(合成方向)、蔗糖合成酶SSⅠ(分解方向)、蔗糖磷酸合成酶(SPS)和柠檬酸分解代谢过程中的胞质顺乌头酸酶(Cyt-ACO)、胞浆异柠檬酸脱氢酶(ICDH)和谷氨酰胺合成酶(GS)的活性。疏果显著促进蔗糖代谢过程中CitSS3在早期的相对表达量和CitSPSs在所有时期的相对表达量,以及柠檬酸分解代谢过程中CitACO3、CitNADP-IDH1、CitACLα1/β、CitGAD1和CitGSs在早中期的相对表达量。【结论】人工疏果主要通过增强蔗糖合成代谢过程中蔗糖磷酸合成酶的酶活来促进果实可溶性糖的积累,提高柠檬酸分解代谢过程中相关酶的活性和基因表达水平,加快柠檬酸降解,从而在提升果实品质中发挥重要作用。
【Background】Beni-Madonna tangor(Citrus nanko×C.amakusa),a hybrid cultivar of citrus,is favored by consumers because of its excellent quality.Fruit thinning is a technique commonly used to improve fruit quality in the process of agricultural production.It can not only significantly increase the fruit volume,but also increase the content of soluble solids in the fruit.However,the specific mechanism of fruit thinning to increase the content of soluble solids in the fruit of Beni-Madonna is still unclear.【Objective】In this study,four-year-old Beni-Madonna tangor plants,with trifoliate orange(Poncirus trifoliata)as a base rootstock and Ponkan as an intermediate stock,were used as experimental materials to explore the specific mechanism of fruit thinning on affecting the change of sugar and acid content in fruit.【Method】Fruit thinning was carried out at the young fruit stage of Beni-Madonna fruit.The horizontal and vertical stems of fruit thinning and non-fruit thinning were measured every half a month or so.The samples were taken back and the single fruit weight and sugar and acid content were measured.The activities of sugar and acid metabolism related enzymes and the relative expression of their corresponding coding genes were measured when the sugar and acid content were significantly different.【Result】The fruit thinning significantly increased the horizontal and vertical stems and single fruit weight of Beni-Madonna fruit in the middle and late stages of fruit growth,significantly accelerated the degradation rate of citrate content,but did not affect the final citrate content at fruit maturity,significantly increased the fruit glucose and sucrose content,but had no significant effect on fructose content.The fruit thinning significantly increased the activities of sucrose synthase SSⅡ(synthesis direction),sucrose synthase SSⅠ(decomposition direction),sucrose phosphate synthase(SPS)in sucrose metabolism and cytosolic aconitase(Cyt-ACO),cytoplasmic isocitrate dehydrogenase(ICDH)and glutamine synt
作者
宋江涛
谌丹丹
公旭晨
商祥明
李春龙
蔡永喜
岳建平
王帅玲
张卜芬
谢宗周
刘继红
SONG JiangTao;SHEN DanDan;GONG XuChen;SHANG XiangMing;LI ChunLong;CAI YongXi;YUEJianPing;WANG ShuaiLing;ZHANG PuFen;XIE ZongZhou;LIU JiHong(Key Laboratory of Horticulture and Botany,Ministry of Education,Huazhong Agricultural University,Wuhan 430070;Yichang Academy of Agricultural Sciences,Yichang 443000,Hubei;Service and Extension Centre of Fruit Trees and Tea in Yidu City,Yidu 443300,Hubei)
出处
《中国农业科学》
CAS
CSCD
北大核心
2022年第23期4688-4701,共14页
Scientia Agricultura Sinica
基金
国家重点研发计划(2019YFD1001402)
湖北省重点研发计划(2020BBA036,2022BBA0073)
湖北省农业创新行动计划。
关键词
疏果
‘爱媛28’
果实品质
蔗糖代谢
柠檬酸代谢
fruit thinning
Beni-Madonna
fruit quality
sucrose metabolism
citric acid metabolism
