摘要
目的通过对比大鼠结肠组织中的Toll样受体4(TLR4)和核苷酸结合寡聚化结构域蛋白2(NOD2)治疗前后水平的变化,从修复肠粘膜屏障的角度探讨四神丸治疗溃疡性结肠炎(ulcerative colitis,UC)的作用机制。方法UC大鼠模型制备采用三硝基苯磺酸/乙醇溶液灌肠法。将40只大鼠随机分为空白组、模型组、柳氮磺嘧啶组(0.36 g/(kg·d))和中药组(5 g/(kg·d)),通过采用HE染色法、RT-qPCR法、SP法和Western Blot法观察结肠组织中TLR4、NOD2的基因和蛋白表达。结果与空白组相比较,模型组大鼠结肠损伤严重,损伤评分显著增高(P<0.01),TLR4、NOD2 mRNA和蛋白表达显著升高(P<0.01,P<0.05);与模型组相比较,各治疗组大鼠结肠损伤有一定程度的恢复,损伤评分显著降低(P<0.01,P<0.05),TLR4、NOD2 mRNA和蛋白表达显著降低(P<0.01,P<0.05)。结论四神丸可以通过提高大鼠的免疫功能,抑制肠道炎症反应,达到治疗UC的目的。
Objective To observe the expression of Toll-like receptor 4(TLR4)and nucleotide binding oligomerization domain protein 2(NOD2)in the colon of a ulcerative colitis(UC)rat model,and to explore the therapeutic mechanism of Sishen pills in the treatment of UC from the perspective of repairing the intestinal mucosal barrier.Methods A UC rat model was prepared by administration of a trinitrobenzenesulfonic acid/ethanol solution enema.Forty rats were randomly divided into four groups,a blank group,model group,salazopyrimidine group(0.36 g/(kg·d)),and Sishen pills group(5 g/(kg·d))The mRNA and protein expressions of TLR4 and NOD2 in colon tissue were detected by HE staining,RT-qPCR,SP immunohistochemistry and Western Blot.Results Compared with the blank group,the colonic mucosal injury score of the model group was significantly greater(P<0.01),and the expressions of TLR4,NOD2 mRNA and protein were significantly greater(P<0.01,P<0.05);Compared with the model group,the scores of colonic mucosal injury in each treatment group were significantly less(P<0.01,P<0.05),and the expressions of TLR4,NOD2 mRNA and protein were significantly less(P<0.01,P<0.05).Conclusions Sishen pills can improve the immune function of rats and inhibit intestinal inflammatory reactions for UC treatment.
作者
何兰娟
邓渊
王燕
朱向东
HE Lanjuan;DENG Yuan;WANG Yan;ZHU Xiangdong(Gansu University of Chinese Medicine,Lanzhou 730101,China;Gansu Provincial Hospital,Lanzhou 730000;Ningxia Medical University,Yinchuan 750004)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2022年第5期613-618,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
国家自然科学基金项目(81360541、81760833、81960826)
兰州市科技发展指导性计划项目(2020-ZD-61)
甘肃省中医药研究中心2020年专项开放课题(zyzx-2020-13)。
