摘要
目的研究脊髓背角星形胶质细胞Cx43半通道在大鼠急性切口痛中的作用。方法成年雄性SD大鼠60只,随机分为对照组(C组)、切口痛组(I组)和Gap19组。Western blot分别检测大鼠急性切口痛术后脊髓背角的Cx43及星形胶质细胞GFAP的表达变化;术前30 min,鞘内应用Gap19测定术后大鼠50%机械缩足阈值(PWT)的变化,免疫荧光检测大鼠脊髓背角的Cx43及GFAP表达的变化,ELISA检测炎症因子的变化。结果与C组相比,I组大鼠脊髓背角Cx43和GFAP表达在术后6 h明显增加,术后3 d和7 d无改变。I组和Gap19组大鼠在切口建立后2 h、6 h、24 h、3 d的PWT明显降低(P<0.01),术后7 d无变化。与I组比,Gap19组PWT在术后2、6、24 h明显增加(P<0.01),3 d和7 d无变化。免疫荧光结果显示,I组术后6 h脊髓GFAP和Cx43表达相较C组增加(P<0.01);与I相比,Gap19组GFAP和Cx43表达明显下降(P<0.05),但术后7 d各组间无统计学差异。ELISA显示,与C组相比,I组脊髓背角炎症因子IL-1β、IL-6以及TNF-α的表达明显增多(P<0.05);与I组相比,Gap19组炎症因子表达则明显下降(P<0.05)。结论特异性抑制Cx43半通道能明显抑制脊髓星形胶质细胞活化和炎症因子表达,减轻急性切口痛大鼠术后痛觉过敏。
Aim To study the role of Cx43 hemichannel in rats with acute incision pain.Methods Adult male rats were randomly divided into normal saline group(C group),incision pain group(I group),Gap19 group.Western blot was used to determine the expression of Cx43 and GFAP at 6 h,3 d and 7 d after paw incision surgery of rats.The rats were intrathecal injected with Gap1930 min before incision surgery,followed by the measurement of the paw withdrawal threshold to mechanical stimuli at several time points.Immunofluorescence was used to detect the expression of Cx43 and GFAP.ELISA was performed to detect the different inflammatory cytokine levels in rats after surgery.Results Compared with group C,the expression of Cx43 and GFAP of rats increased significantly 6 h after incision surgery in group I,while their expression at postoperative 3 d and 7 d showed no obvious alteration.Compared with the preoperative baseline,the mechanical paw withdrawal threshold in I group and Gap19 group rats was significantly reduced at 2 h,6 h,24 h and 3 d post-incision(P<0.01),but it had no obvious change on postoperative 7 d.Compared with group I,the mechanical paw withdrawal threshold in rats of Gap19 group increased 2 h,6 h,24 h post-incision,while it showed no statistical difference on 3 d and 7 d post-incision.Compared with group C,immunofluorescence results showed that the expression of GFAP and Cx43 in spinal cord dorsal horn astrocytes also increased significantly in Group I 6 h after incision surgery.Compared with group I,GFAP and the expression of Cx43 were markedly reduced in Gap19 group.There was no statistical difference on 7 d post-incision.Compared with those in group C,the inflammation factors including IL-1β,IL-6 and TNF-αobviously increased in group I.However,in contrary to the rats in group I,the rats pretreated with gap19 showed increased expression of Cx43 and GFAP in spinal cord dorsal horn.Conclusions Specific inhibition of Cx43 hemichannel significantly suppresses astrocyte activation and alters the inflammatory microenv
作者
颜茹芳
李佳璟
查进
黄焕森
汪灵芝
YAN Ru-fang;LI Jia-jing;ZHA Jin;HUANG Huan-sen;WANG Ling-zhi(Dept of Anesthesiology,the Second Affiliated Hospital of Guangzhou Medical University,Guangdong 510260,China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2022年第11期1655-1661,共7页
Chinese Pharmacological Bulletin
基金
广东省基础与应用基础研究基金资助项目(No.2020 A151501070)。
