摘要
以七叶莲为试材,采用纤维素酶协同超声辅助提取七叶莲多酚,通过单因素试验和响应面试验优化提取条件,研究了七叶莲多酚对羟基自由基(·OH)、DPPH自由基(DPPH·)和亚硝酸盐(NO_(2)^(-))的清除能力,以期为综合开发利用七叶莲多酚提供参考依据。结果表明:七叶莲多酚最佳提取工艺条件为乙醇体积分数40%,料液比1:35g·mL^(-1),酶解时间35min,超声功率350 W,酶解温度50℃,纤维素酶用量3.0%(以七叶莲干粉质量为基准,下同);在该条件下,七叶莲多酚提取量为(15.52±0.824)mg·g^(-1),与预测值(15.61mg·g^(-1))接近;七叶莲多酚对·OH、DPPH·和NO_(2)^(-)有较强的清除能力,且随多酚质量浓度的增大而逐渐增强;七叶莲多酚质量浓度为0.137mg·mL^(-1)时,对·OH、DPPH·和NO_(2)^(-)的清除率分别为54.33%、91.14%和61.42%。采用响应面法优化的多酚提取工艺参数准确可靠,可用于七叶莲多酚提取;七叶莲多酚具有较强的体外抗氧化能力,可作为食品及医药的天然抗氧化剂资源进行开发利用。
Taking Schefflera arboricola Hayata as the test material,extraction of polyphenols from Schefflera arboricola Hayata by cellulase combined with ultrasound was studied,the extraction conditions were optimized by univariate and response tests and the scavenging capacity of polyphenols fromSchefflera arboricola Hayata on hydroxyl radicals(·OH),DPPH radicals(DPPH·)and nitrite(NO_(2)^(-))was also studied,in order to provide reference for the comprehensive development and utilization of Schefflera arboricola Hayata polyphenols.The results showed that the optimum extraction conditions of polyphenols fromSchefflera arboricola Hayata were ethanol volume fraction 40%,solid-liquid ratio 1:35g·mL^(-1),enzymolysis hydrolysis time 35minutes,ultrasonic power 350 W,enzymolysis temperature 50℃,cellulase dosage 3.0%(with the quality of Schefflera arboricola Hayata,the same below).Under these conditions,the extraction amount of polyphenols fromSchefflera arboricola Hayata was(15.52±0.824)mg·g^(-1),which was close to the predicted value(15.61mg·g^(-1)).Schefflera arboricola Hayata polyphenols showed strong scavenging effects on hydroxyl radicals,DPPH radicals and nitrite,and increased with the increase of polyphenols concentration.When the concentration of Schefflera arboricola Hayata polyphenols was 0.137mg·mL^(-1),the clearance rates of·OH,DPPH·and NO_(2)^(-) were 54.33%,91.14%and 61.42%respectively.The extraction process parameters optimized by response method were accurate and reliable,and could be used for the extraction of polyphenols fromSchefflera arboricola Hayata.Schefflera arboricola Hayata polyphenols had strong antioxidant capacity in vitro and could be used as natural antioxidant resources in food and medicine.
作者
杨申明
宁娜
杨潇
马金林
李正江
王振吉
YANG Shenming;NING Na;YANG Xiao;MA Jinlin;LI Zhengjiang;WANG Zhenji(Department of Resources,Environment and Chemistry,Chuxiong Normal University,Chuxiong,Yunnan 675000)
出处
《北方园艺》
CAS
北大核心
2022年第18期90-98,共9页
Northern Horticulture
基金
云南省中青年学术和技术带头人后备人才资助项目(2019HB055)
云南省“高层次人才培养支持计划”青年拔尖人才资助项目(YNWR-QNBJ-2020-104)
“彝乡科技领军人才”资助项目
楚雄师范学院开放实验室资助项目。
关键词
七叶莲
多酚
超声提取
响应面法
抗氧化性
Schefflera arboricola Hayata
polyphenols
ultrasound extraction
response surface methodology
antioxidant activity
